Differentially expressed miRNAs in retinoblastoma

Joshua Martin, Paul Bryar, Marilyn Mets, Joanna Weinstein, Aunica Jones, Alissa Martin, Elio F. Vanin, Denise Scholtens, Fabricio F. Costa, Marcelo B. Soares, Nikia A. Laurie*

*Corresponding author for this work

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

MicroRNAs (miRNAs) are short non-coding RNA transcripts that have the ability to regulate the expression of target genes, and have been shown to influence the development of various tumors. The purpose of our study is to identify aberrantly expressed miRNAs in retinoblastoma for the discovery of potential therapeutic targets for this disease, and to gain a greater understanding of the mechanisms driving retinoblastoma progression. We report 41 differentially expressed miRNAs (p< 0.05) in 12 retinoblastomas as compared to three normal human retinae. Of these miRNAs, many are newly identified as being differentially expressed in retinoblastoma. Further, we report the validations of five of the most downregulated miRNAs in primary human retinoblastomas (p<0.05), human retinoblastoma cell lines, and mouse retinoblastoma cell lines. This serves as the largest and most comprehensive retinoblastoma miRNA analysis to date with corresponding clinical and pathological characteristics. This is an essential step in the discovery of miRNAs associated with retinoblastoma progression, and in the identification of potential therapeutic targets for this disease.

Original languageEnglish (US)
Pages (from-to)294-299
Number of pages6
JournalGene
Volume512
Issue number2
DOIs
StatePublished - Jan 10 2013

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Keywords

  • MicroRNA
  • Retina
  • Taqman low density array

ASJC Scopus subject areas

  • Genetics

Cite this

Martin, J., Bryar, P., Mets, M., Weinstein, J., Jones, A., Martin, A., Vanin, E. F., Scholtens, D., Costa, F. F., Soares, M. B., & Laurie, N. A. (2013). Differentially expressed miRNAs in retinoblastoma. Gene, 512(2), 294-299. https://doi.org/10.1016/j.gene.2012.09.129