Differentially expressed miRNAs in retinoblastoma

Joshua Martin; Paul Bryar; Marilyn Mets; Joanna Weinstein; Aunica Jones; Alissa Martin; Elio F. Vanin; Denise Scholtens; Fabricio F. Costa; Marcelo B. Soares; Nikia A. Laurie

doi:10.1016/j.gene.2012.09.129

Differentially expressed miRNAs in retinoblastoma

Joshua Martin, Paul Bryar, Marilyn Mets, Joanna Weinstein, Aunica Jones, Alissa Martin, Elio F. Vanin, Denise Scholtens, Fabricio F. Costa, Marcelo B. Soares, Nikia A. Laurie^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

MicroRNAs (miRNAs) are short non-coding RNA transcripts that have the ability to regulate the expression of target genes, and have been shown to influence the development of various tumors. The purpose of our study is to identify aberrantly expressed miRNAs in retinoblastoma for the discovery of potential therapeutic targets for this disease, and to gain a greater understanding of the mechanisms driving retinoblastoma progression. We report 41 differentially expressed miRNAs (p< 0.05) in 12 retinoblastomas as compared to three normal human retinae. Of these miRNAs, many are newly identified as being differentially expressed in retinoblastoma. Further, we report the validations of five of the most downregulated miRNAs in primary human retinoblastomas (p<0.05), human retinoblastoma cell lines, and mouse retinoblastoma cell lines. This serves as the largest and most comprehensive retinoblastoma miRNA analysis to date with corresponding clinical and pathological characteristics. This is an essential step in the discovery of miRNAs associated with retinoblastoma progression, and in the identification of potential therapeutic targets for this disease.

Original language	English (US)
Pages (from-to)	294-299
Number of pages	6
Journal	Gene
Volume	512
Issue number	2
DOIs	https://doi.org/10.1016/j.gene.2012.09.129
State	Published - Jan 10 2013

Funding

The authors would like to thank Aaliyah Hamidullah and Jatuphon Chaiseesiri for technical assistance and Marianne Reed and Elaine Hanrahan for administrative assistance. The authors would also like to thank Ann Harris and Christine DiDonato for helpful discussions. This work was supported by the Illinois Department of Public Aid – Excellence in Academic Medicine Award (to N.A.L.), the Zell Family Foundation (to N.A.L.), Bear Necessities Pediatric Cancer Foundation (to N.A.L.), Research to Prevent Blindness (to P.B), the Maeve McNicholas Memorial Foundation (to F.F.C) and the Robert H. Lurie Comprehensive Cancer Center support grant P30 CA060553 .

Keywords

MicroRNA
Retina
Taqman low density array

ASJC Scopus subject areas

Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.gene.2012.09.129

Cite this

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title = "Differentially expressed miRNAs in retinoblastoma",

abstract = "MicroRNAs (miRNAs) are short non-coding RNA transcripts that have the ability to regulate the expression of target genes, and have been shown to influence the development of various tumors. The purpose of our study is to identify aberrantly expressed miRNAs in retinoblastoma for the discovery of potential therapeutic targets for this disease, and to gain a greater understanding of the mechanisms driving retinoblastoma progression. We report 41 differentially expressed miRNAs (p< 0.05) in 12 retinoblastomas as compared to three normal human retinae. Of these miRNAs, many are newly identified as being differentially expressed in retinoblastoma. Further, we report the validations of five of the most downregulated miRNAs in primary human retinoblastomas (p<0.05), human retinoblastoma cell lines, and mouse retinoblastoma cell lines. This serves as the largest and most comprehensive retinoblastoma miRNA analysis to date with corresponding clinical and pathological characteristics. This is an essential step in the discovery of miRNAs associated with retinoblastoma progression, and in the identification of potential therapeutic targets for this disease.",

keywords = "MicroRNA, Retina, Taqman low density array",

author = "Joshua Martin and Paul Bryar and Marilyn Mets and Joanna Weinstein and Aunica Jones and Alissa Martin and Vanin, {Elio F.} and Denise Scholtens and Costa, {Fabricio F.} and Soares, {Marcelo B.} and Laurie, {Nikia A.}",

note = "Funding Information: The authors would like to thank Aaliyah Hamidullah and Jatuphon Chaiseesiri for technical assistance and Marianne Reed and Elaine Hanrahan for administrative assistance. The authors would also like to thank Ann Harris and Christine DiDonato for helpful discussions. This work was supported by the Illinois Department of Public Aid – Excellence in Academic Medicine Award (to N.A.L.), the Zell Family Foundation (to N.A.L.), Bear Necessities Pediatric Cancer Foundation (to N.A.L.), Research to Prevent Blindness (to P.B), the Maeve McNicholas Memorial Foundation (to F.F.C) and the Robert H. Lurie Comprehensive Cancer Center support grant P30 CA060553 .",

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AU - Martin, Joshua

AU - Bryar, Paul

AU - Mets, Marilyn

AU - Weinstein, Joanna

AU - Jones, Aunica

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AU - Vanin, Elio F.

AU - Scholtens, Denise

AU - Costa, Fabricio F.

AU - Soares, Marcelo B.

AU - Laurie, Nikia A.

N1 - Funding Information: The authors would like to thank Aaliyah Hamidullah and Jatuphon Chaiseesiri for technical assistance and Marianne Reed and Elaine Hanrahan for administrative assistance. The authors would also like to thank Ann Harris and Christine DiDonato for helpful discussions. This work was supported by the Illinois Department of Public Aid – Excellence in Academic Medicine Award (to N.A.L.), the Zell Family Foundation (to N.A.L.), Bear Necessities Pediatric Cancer Foundation (to N.A.L.), Research to Prevent Blindness (to P.B), the Maeve McNicholas Memorial Foundation (to F.F.C) and the Robert H. Lurie Comprehensive Cancer Center support grant P30 CA060553 .

PY - 2013/1/10

Y1 - 2013/1/10

N2 - MicroRNAs (miRNAs) are short non-coding RNA transcripts that have the ability to regulate the expression of target genes, and have been shown to influence the development of various tumors. The purpose of our study is to identify aberrantly expressed miRNAs in retinoblastoma for the discovery of potential therapeutic targets for this disease, and to gain a greater understanding of the mechanisms driving retinoblastoma progression. We report 41 differentially expressed miRNAs (p< 0.05) in 12 retinoblastomas as compared to three normal human retinae. Of these miRNAs, many are newly identified as being differentially expressed in retinoblastoma. Further, we report the validations of five of the most downregulated miRNAs in primary human retinoblastomas (p<0.05), human retinoblastoma cell lines, and mouse retinoblastoma cell lines. This serves as the largest and most comprehensive retinoblastoma miRNA analysis to date with corresponding clinical and pathological characteristics. This is an essential step in the discovery of miRNAs associated with retinoblastoma progression, and in the identification of potential therapeutic targets for this disease.

AB - MicroRNAs (miRNAs) are short non-coding RNA transcripts that have the ability to regulate the expression of target genes, and have been shown to influence the development of various tumors. The purpose of our study is to identify aberrantly expressed miRNAs in retinoblastoma for the discovery of potential therapeutic targets for this disease, and to gain a greater understanding of the mechanisms driving retinoblastoma progression. We report 41 differentially expressed miRNAs (p< 0.05) in 12 retinoblastomas as compared to three normal human retinae. Of these miRNAs, many are newly identified as being differentially expressed in retinoblastoma. Further, we report the validations of five of the most downregulated miRNAs in primary human retinoblastomas (p<0.05), human retinoblastoma cell lines, and mouse retinoblastoma cell lines. This serves as the largest and most comprehensive retinoblastoma miRNA analysis to date with corresponding clinical and pathological characteristics. This is an essential step in the discovery of miRNAs associated with retinoblastoma progression, and in the identification of potential therapeutic targets for this disease.

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Differentially expressed miRNAs in retinoblastoma

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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