Diffuse reflectance spectroscopy of human adenomatous colon polyps in vivo

George Zonios, Lev T. Perelman, Vadim Backman, Ramasamy Manoharan, Maryann Fitzmaurice, Jacques Van Dam, Michael S. Feld

Research output: Contribution to journalArticlepeer-review

506 Scopus citations


Diffuse reflectance spectra were collected from adenomatous colon polyps (cancer precursors) and normal colonic mucosa of patients undergoing colonoscopy. We analyzed the data by using an analytical light diffusion model, which was tested and validated on a physical tissue model composed of polystyrene beads and hemoglobin. Four parameters were obtained: hemoglobin concentration, hemoglobin oxygen saturation, effective scatterer density, and effective scatterer size. Normal and adenomatous tissue sites exhibited differences in hemoglobin concentration and, on average, in effective scatterer size, which were in general agreement with other studies that employ standard methods. These results suggest that diffuse reflectance can be used to obtain tissue information about tissue structure and composition in vivo.

Original languageEnglish (US)
Pages (from-to)6628-6637
Number of pages10
JournalApplied optics
Issue number31
StatePublished - Oct 20 1999

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering


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