Digital analysis yields more reliable and accurate measures of dermal and epidermal thickness in histologically processed specimens compared to traditional methods

Sergey Y. Turin; Joanna K. Ledwon; Hanna Bae; Adrian Buganza-Tepole; Jolanta Topczewska; Arun K. Gosain

doi:10.1111/exd.13534

Digital analysis yields more reliable and accurate measures of dermal and epidermal thickness in histologically processed specimens compared to traditional methods

Sergey Y. Turin, Joanna K. Ledwon, Hanna Bae, Adrian Buganza-Tepole, Jolanta Topczewska, Arun K. Gosain^*

^*Corresponding author for this work

Surgery

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Changes in the thickness of the dermis and epidermis have been described in the scenario of tissue expansion as well as inflammatory skin processes (psoriasis, contact hypersensitivity and so on). These changes have previously been quantified using ocular micrometers to obtain and then average a limited number of spot measurements, leading to suboptimal accuracy. We describe a rapid method of using freely available ImageJ software to analyze digitized images of fixed skin specimens. By determining the cross-sectional area and surface length of a skin layer, a simple calculation produces more accurate and reproducible measurements of its thickness compared to historical methods, with excellent inter-rater reliability.

Original language	English (US)
Pages (from-to)	687-690
Number of pages	4
Journal	Experimental Dermatology
Volume	27
Issue number	6
DOIs	https://doi.org/10.1111/exd.13534
State	Published - Jun 2018

Keywords

digital
expansion
in vitro
layer
stretch

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Dermatology

Access to Document

10.1111/exd.13534

Cite this

@article{c6f1e304ca7543fab851b115b300bc18,

title = "Digital analysis yields more reliable and accurate measures of dermal and epidermal thickness in histologically processed specimens compared to traditional methods",

abstract = "Changes in the thickness of the dermis and epidermis have been described in the scenario of tissue expansion as well as inflammatory skin processes (psoriasis, contact hypersensitivity and so on). These changes have previously been quantified using ocular micrometers to obtain and then average a limited number of spot measurements, leading to suboptimal accuracy. We describe a rapid method of using freely available ImageJ software to analyze digitized images of fixed skin specimens. By determining the cross-sectional area and surface length of a skin layer, a simple calculation produces more accurate and reproducible measurements of its thickness compared to historical methods, with excellent inter-rater reliability.",

keywords = "digital, expansion, in vitro, layer, stretch",

author = "Turin, {Sergey Y.} and Ledwon, {Joanna K.} and Hanna Bae and Adrian Buganza-Tepole and Jolanta Topczewska and Gosain, {Arun K.}",

note = "Publisher Copyright: {\textcopyright} 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd",

year = "2018",

month = jun,

doi = "10.1111/exd.13534",

language = "English (US)",

volume = "27",

pages = "687--690",

journal = "Experimental Dermatology",

issn = "0906-6705",

publisher = "Wiley-Blackwell",

number = "6",

}

TY - JOUR

T1 - Digital analysis yields more reliable and accurate measures of dermal and epidermal thickness in histologically processed specimens compared to traditional methods

AU - Turin, Sergey Y.

AU - Ledwon, Joanna K.

AU - Bae, Hanna

AU - Buganza-Tepole, Adrian

AU - Topczewska, Jolanta

AU - Gosain, Arun K.

PY - 2018/6

Y1 - 2018/6

N2 - Changes in the thickness of the dermis and epidermis have been described in the scenario of tissue expansion as well as inflammatory skin processes (psoriasis, contact hypersensitivity and so on). These changes have previously been quantified using ocular micrometers to obtain and then average a limited number of spot measurements, leading to suboptimal accuracy. We describe a rapid method of using freely available ImageJ software to analyze digitized images of fixed skin specimens. By determining the cross-sectional area and surface length of a skin layer, a simple calculation produces more accurate and reproducible measurements of its thickness compared to historical methods, with excellent inter-rater reliability.

AB - Changes in the thickness of the dermis and epidermis have been described in the scenario of tissue expansion as well as inflammatory skin processes (psoriasis, contact hypersensitivity and so on). These changes have previously been quantified using ocular micrometers to obtain and then average a limited number of spot measurements, leading to suboptimal accuracy. We describe a rapid method of using freely available ImageJ software to analyze digitized images of fixed skin specimens. By determining the cross-sectional area and surface length of a skin layer, a simple calculation produces more accurate and reproducible measurements of its thickness compared to historical methods, with excellent inter-rater reliability.

KW - digital

KW - expansion

KW - in vitro

KW - layer

KW - stretch

UR - http://www.scopus.com/inward/record.url?scp=85044954947&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85044954947&partnerID=8YFLogxK

U2 - 10.1111/exd.13534

DO - 10.1111/exd.13534

M3 - Article

C2 - 29573291

AN - SCOPUS:85044954947

SN - 0906-6705

VL - 27

SP - 687

EP - 690

JO - Experimental Dermatology

JF - Experimental Dermatology

IS - 6

ER -

Digital analysis yields more reliable and accurate measures of dermal and epidermal thickness in histologically processed specimens compared to traditional methods

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this