Digital sensing and sizing of vesicular stomatitis virus pseudotypes in complex media: A model for ebola and marburg detection

George G. Daaboul, Carlos A. Lopez, Jyothsna Chinnala, Bennett B. Goldberg, John H. Connor, M. Selim Ünlü*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

80 Scopus citations


Rapid, sensitive, and direct label-free capture and characterization of nanoparticles from complex media such as blood or serum will broadly impact medicine and the life sciences. We demonstrate identification of virus particles in complex samples for replication-competent wild-type vesicular stomatitis virus (VSV), defective VSV, and Ebola- and Marburg-pseudotyped VSV with high sensitivity and specificity. Size discrimination of the imaged nanoparticles (virions) allows differentiation between modified viruses having different genome lengths and facilitates a reduction in the counting of nonspecifically bound particles to achieve a limit-of-detection (LOD) of 5 × 10 3 pfu/mL for the Ebola and Marburg VSV pseudotypes. We demonstrate the simultaneous detection of multiple viruses in a single sample (composed of serum or whole blood) for screening applications and uncompromised detection capabilities in samples contaminated with high levels of bacteria. By employing affinity-based capture, size discrimination, and a digital detection scheme to count single virus particles, we show that a robust and sensitive virus/nanoparticle sensing assay can be established for targets in complex samples. The nanoparticle microscopy system is termed the Single Particle Interferometric Reflectance Imaging Sensor (SP-IRIS) and is capable of high-throughput and rapid sizing of large numbers of biological nanoparticles on an antibody microarray for research and diagnostic applications.

Original languageEnglish (US)
Pages (from-to)6047-6055
Number of pages9
JournalACS nano
Issue number6
StatePublished - Jun 24 2014


  • biosensor
  • label free
  • single virus
  • viral hemorrhagic fevers

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)
  • Materials Science(all)


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