Dihydropyridine sensitive calcium channels in a smooth muscle cell line

Sathapana Kongsamut; Stephen B. Freedman; Richard J. Miller

doi:10.1016/S0006-291X(85)80127-3

Dihydropyridine sensitive calcium channels in a smooth muscle cell line

Sathapana Kongsamut^*, Stephen B. Freedman, Richard J. Miller

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

The pharmacological properties of voltage sensitive calcium channels (VSCC) were examined in a rat aortic smooth muscle cell line (A10). The inorganic VSCC blockers Co²⁺ and Cd²⁺ blocked ⁴⁵Ca²⁺ uptake into these cells in both 5 mM K⁺ and 50 mM K⁺ (depolarizing) conditions. The organic VSCC antagonists nitrendipine, nimodipine, D-600 and diltiazem also blocked ⁴⁵Ca²⁺ uptake at low concentrations. The relative potencies of blockade were similar to those found in intact vascular smooth muscle. The VSCC "agonist" BAY K8644 enhanced ⁴⁵Ca²⁺ uptake and this effect could be reversed by nitrendipine. These results indicate that A10 cells possess VSCC and that these VSCC behave similarly to those in authentic smooth muscle.

Original language	English (US)
Pages (from-to)	71-79
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	127
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(85)80127-3
State	Published - Feb 28 1985

Funding

1. This work was supported by NIH grants PHS DA-02121 and PHS DA-02575 to P.J.M. S.B.F. was a NATO (UK) Fellow. 2. Present address: Neuroscience Research Centre, Merck Sharp

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/S0006-291X(85)80127-3

Cite this

@article{49771eb45df64e0188a3ab3933900e5a,

title = "Dihydropyridine sensitive calcium channels in a smooth muscle cell line",

abstract = "The pharmacological properties of voltage sensitive calcium channels (VSCC) were examined in a rat aortic smooth muscle cell line (A10). The inorganic VSCC blockers Co2+ and Cd2+ blocked 45Ca2+ uptake into these cells in both 5 mM K+ and 50 mM K+ (depolarizing) conditions. The organic VSCC antagonists nitrendipine, nimodipine, D-600 and diltiazem also blocked 45Ca2+ uptake at low concentrations. The relative potencies of blockade were similar to those found in intact vascular smooth muscle. The VSCC {"}agonist{"} BAY K8644 enhanced 45Ca2+ uptake and this effect could be reversed by nitrendipine. These results indicate that A10 cells possess VSCC and that these VSCC behave similarly to those in authentic smooth muscle.",

author = "Sathapana Kongsamut and Freedman, {Stephen B.} and Miller, {Richard J.}",

note = "Funding Information: 1. This work was supported by NIH grants PHS DA-02121 and PHS DA-02575 to P.J.M. S.B.F. was a NATO (UK) Fellow. 2. Present address: Neuroscience Research Centre, Merck Sharp",

year = "1985",

month = feb,

day = "28",

doi = "10.1016/S0006-291X(85)80127-3",

language = "English (US)",

volume = "127",

pages = "71--79",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Dihydropyridine sensitive calcium channels in a smooth muscle cell line

AU - Kongsamut, Sathapana

AU - Freedman, Stephen B.

AU - Miller, Richard J.

N1 - Funding Information: 1. This work was supported by NIH grants PHS DA-02121 and PHS DA-02575 to P.J.M. S.B.F. was a NATO (UK) Fellow. 2. Present address: Neuroscience Research Centre, Merck Sharp

PY - 1985/2/28

Y1 - 1985/2/28

N2 - The pharmacological properties of voltage sensitive calcium channels (VSCC) were examined in a rat aortic smooth muscle cell line (A10). The inorganic VSCC blockers Co2+ and Cd2+ blocked 45Ca2+ uptake into these cells in both 5 mM K+ and 50 mM K+ (depolarizing) conditions. The organic VSCC antagonists nitrendipine, nimodipine, D-600 and diltiazem also blocked 45Ca2+ uptake at low concentrations. The relative potencies of blockade were similar to those found in intact vascular smooth muscle. The VSCC "agonist" BAY K8644 enhanced 45Ca2+ uptake and this effect could be reversed by nitrendipine. These results indicate that A10 cells possess VSCC and that these VSCC behave similarly to those in authentic smooth muscle.

AB - The pharmacological properties of voltage sensitive calcium channels (VSCC) were examined in a rat aortic smooth muscle cell line (A10). The inorganic VSCC blockers Co2+ and Cd2+ blocked 45Ca2+ uptake into these cells in both 5 mM K+ and 50 mM K+ (depolarizing) conditions. The organic VSCC antagonists nitrendipine, nimodipine, D-600 and diltiazem also blocked 45Ca2+ uptake at low concentrations. The relative potencies of blockade were similar to those found in intact vascular smooth muscle. The VSCC "agonist" BAY K8644 enhanced 45Ca2+ uptake and this effect could be reversed by nitrendipine. These results indicate that A10 cells possess VSCC and that these VSCC behave similarly to those in authentic smooth muscle.

UR - http://www.scopus.com/inward/record.url?scp=0021924572&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021924572&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(85)80127-3

DO - 10.1016/S0006-291X(85)80127-3

M3 - Article

C2 - 2579654

AN - SCOPUS:0021924572

SN - 0006-291X

VL - 127

SP - 71

EP - 79

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Dihydropyridine sensitive calcium channels in a smooth muscle cell line

Abstract

Funding

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this