Dipeptide Nanostructure Assembly and Dynamics via in Situ Liquid-Phase Electron Microscopy

Karthikeyan Gnanasekaran, Joanna Korpanty, Or Berger, Nicholas Hampu, Michal Halperin-Sternfeld, Dana Cohen-Gerassi, Lihi Adler-Abramovich, Nathan C. Gianneschi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


In this paper, we report the in situ growth of FF nanotubes examined via liquid-cell transmission electron microscopy (LCTEM). This direct, high spatial, and temporal resolution imaging approach allowed us to observe the growth of peptide-based nanofibrillar structures through directional elongation. Furthermore, the radial growth profile of FF nanotubes through the addition of monomers perpendicular to the tube axis has been observed in real-time with sufficient resolution to directly observe the increase in diameter. Our study demonstrates that the kinetics, dynamics, structure formation, and assembly mechanism of these supramolecular assemblies can be directly monitored using LCTEM. The performance of the peptides and the assemblies they form can be verified and evaluated using post-mortem techniques including time-of-flight secondary ion mass spectrometry (ToF-SIMS).

Original languageEnglish (US)
Pages (from-to)16542-16551
Number of pages10
JournalACS nano
Issue number10
StatePublished - Oct 26 2021


  • ToF-SIMS
  • dipeptides
  • diphenylalanine
  • liquid-cell TEM
  • self-assembly

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)


Dive into the research topics of 'Dipeptide Nanostructure Assembly and Dynamics via in Situ Liquid-Phase Electron Microscopy'. Together they form a unique fingerprint.

Cite this