TY - JOUR
T1 - Dipeptide Nanostructure Assembly and Dynamics via in Situ Liquid-Phase Electron Microscopy
AU - Gnanasekaran, Karthikeyan
AU - Korpanty, Joanna
AU - Berger, Or
AU - Hampu, Nicholas
AU - Halperin-Sternfeld, Michal
AU - Cohen-Gerassi, Dana
AU - Adler-Abramovich, Lihi
AU - Gianneschi, Nathan C.
N1 - Funding Information:
This research used the EPIC facility of Northwestern University’s NU ANCE Center, which has received support from the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS-1542205); the MRSEC program (NSF DMR-1720139) at the Materials Research Center; the International Institute for Nanotechnology (IIN); the Keck Foundation; and the State of Illinois, through the IIN. Research reported in this publication was supported in part by instrumentation provided by the Office of The Director, National Institutes of Health of the National Institutes of Health under Award Number S10OD026871. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Furthermore, the authors would like to thank the Army Research Office (W911NF-17-1-0326, W911NF-18-1-0359, MURI W911NF-15-1-0568) and the National Science Foundation (CHE-MSN 1905270) for support. K.G. is appreciative of a postdoctoral fellowship from the Human Frontier Science Program (LT000869/2018-C). J.K. gratefully acknowledges support from the Ryan Fellowship and the International Institute for Nanotechnology at Northwestern University. The authors acknowledge a Clore scholarship (M.H.-S), BSF (grant No. 2018102) (M.H.-S), GRTF travel grant (M.H.-S), and the Israel Science Foundation (grant No. 1732/17) (L.A.-A.) for support. Portions of this work were performed at the DuPont-Northwestern-Dow Collaborative Access Team (DND-CAT) located at Sector 5 of the Advanced Photon Source (APS). DND-CAT is supported by Northwestern University, E.I. DuPont de Nemours & Co., and The Dow Chemical Company. This research used resources of the APS, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE-AC02-06CH11357. Data were collected using an instrument funded by the NSF under Award Number 0960140. The authors would like to acknowledge the Gianneschi and Adler-Abramovich research groups for fruitful discussions.
Publisher Copyright:
© 2021 American Chemical Society.
PY - 2021/10/26
Y1 - 2021/10/26
N2 - In this paper, we report the in situ growth of FF nanotubes examined via liquid-cell transmission electron microscopy (LCTEM). This direct, high spatial, and temporal resolution imaging approach allowed us to observe the growth of peptide-based nanofibrillar structures through directional elongation. Furthermore, the radial growth profile of FF nanotubes through the addition of monomers perpendicular to the tube axis has been observed in real-time with sufficient resolution to directly observe the increase in diameter. Our study demonstrates that the kinetics, dynamics, structure formation, and assembly mechanism of these supramolecular assemblies can be directly monitored using LCTEM. The performance of the peptides and the assemblies they form can be verified and evaluated using post-mortem techniques including time-of-flight secondary ion mass spectrometry (ToF-SIMS).
AB - In this paper, we report the in situ growth of FF nanotubes examined via liquid-cell transmission electron microscopy (LCTEM). This direct, high spatial, and temporal resolution imaging approach allowed us to observe the growth of peptide-based nanofibrillar structures through directional elongation. Furthermore, the radial growth profile of FF nanotubes through the addition of monomers perpendicular to the tube axis has been observed in real-time with sufficient resolution to directly observe the increase in diameter. Our study demonstrates that the kinetics, dynamics, structure formation, and assembly mechanism of these supramolecular assemblies can be directly monitored using LCTEM. The performance of the peptides and the assemblies they form can be verified and evaluated using post-mortem techniques including time-of-flight secondary ion mass spectrometry (ToF-SIMS).
KW - ToF-SIMS
KW - dipeptides
KW - diphenylalanine
KW - liquid-cell TEM
KW - self-assembly
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U2 - 10.1021/acsnano.1c06130
DO - 10.1021/acsnano.1c06130
M3 - Article
C2 - 34623126
AN - SCOPUS:85117779414
SN - 1936-0851
VL - 15
SP - 16542
EP - 16551
JO - ACS Nano
JF - ACS Nano
IS - 10
ER -