Diphenylhydantoin attenuates hypoxia-induced release of [3H]glutamate from rat hippocampal slices

Pamela E. Potter*, Paul Detwiler, Beverley Thorne, Joseph R. Moskal

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The ability of diphenylhydantoin (DPH) to prevent hypoxia-induced [3H]glutamate release was examined in perfused rat hippocampal slices. Hypoxia (25 min; 95% N2/5% CO2) caused a prolonged release of [3H]glutamate, which was reduced significantly if DPH (20 μM) was present from the beginning of the perfusion. Perfusion with oxygenated medium (reoxygenation) following hypoxia also caused a pronounced release of glutamate. A therapeutic concentration of DPH, added before, during, or after hypoxia, decreased this release of glutamate. These results suggest that DPH may protect against glutamate-mediated neurotoxicity associated with stroke.

Original languageEnglish (US)
Pages (from-to)127-130
Number of pages4
JournalBrain research
Volume558
Issue number1
DOIs
StatePublished - Aug 30 1991

Keywords

  • Diphenylhydantoin
  • Glutamate
  • Hippocampus
  • Hypoxia
  • Ischemia
  • Phenytoin
  • Stroke

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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