Diphenylhydantoin attenuates hypoxia-induced release of [3H]glutamate from rat hippocampal slices

Pamela E. Potter; Paul Detwiler; Beverley Thorne; Joseph R. Moskal

doi:10.1016/0006-8993(91)90728-E

Diphenylhydantoin attenuates hypoxia-induced release of [³H]glutamate from rat hippocampal slices

Pamela E. Potter^*, Paul Detwiler, Beverley Thorne, Joseph R. Moskal

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The ability of diphenylhydantoin (DPH) to prevent hypoxia-induced [³H]glutamate release was examined in perfused rat hippocampal slices. Hypoxia (25 min; 95% N₂/5% CO₂) caused a prolonged release of [³H]glutamate, which was reduced significantly if DPH (20 μM) was present from the beginning of the perfusion. Perfusion with oxygenated medium (reoxygenation) following hypoxia also caused a pronounced release of glutamate. A therapeutic concentration of DPH, added before, during, or after hypoxia, decreased this release of glutamate. These results suggest that DPH may protect against glutamate-mediated neurotoxicity associated with stroke.

Original language	English (US)
Pages (from-to)	127-130
Number of pages	4
Journal	Brain research
Volume	558
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(91)90728-E
State	Published - Aug 30 1991

Keywords

Diphenylhydantoin
Glutamate
Hippocampus
Hypoxia
Ischemia
Phenytoin
Stroke

ASJC Scopus subject areas

Clinical Neurology
Molecular Biology
General Neuroscience
Developmental Biology

Access to Document

10.1016/0006-8993(91)90728-E

Cite this

@article{acf0a52545324f6a86b60c7ec7ffb96f,

title = "Diphenylhydantoin attenuates hypoxia-induced release of [3H]glutamate from rat hippocampal slices",

abstract = "The ability of diphenylhydantoin (DPH) to prevent hypoxia-induced [3H]glutamate release was examined in perfused rat hippocampal slices. Hypoxia (25 min; 95% N2/5% CO2) caused a prolonged release of [3H]glutamate, which was reduced significantly if DPH (20 μM) was present from the beginning of the perfusion. Perfusion with oxygenated medium (reoxygenation) following hypoxia also caused a pronounced release of glutamate. A therapeutic concentration of DPH, added before, during, or after hypoxia, decreased this release of glutamate. These results suggest that DPH may protect against glutamate-mediated neurotoxicity associated with stroke.",

keywords = "Diphenylhydantoin, Glutamate, Hippocampus, Hypoxia, Ischemia, Phenytoin, Stroke",

author = "Potter, {Pamela E.} and Paul Detwiler and Beverley Thorne and Moskal, {Joseph R.}",

note = "Funding Information: This work was supported by the Health Foundation. B.T. is the recipient of an IBRO/UNESCO fellowship, P.E.E would like to",

year = "1991",

month = aug,

day = "30",

doi = "10.1016/0006-8993(91)90728-E",

language = "English (US)",

volume = "558",

pages = "127--130",

journal = "Brain research",

issn = "0006-8993",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Diphenylhydantoin attenuates hypoxia-induced release of [3H]glutamate from rat hippocampal slices

AU - Potter, Pamela E.

AU - Detwiler, Paul

AU - Thorne, Beverley

AU - Moskal, Joseph R.

N1 - Funding Information: This work was supported by the Health Foundation. B.T. is the recipient of an IBRO/UNESCO fellowship, P.E.E would like to

PY - 1991/8/30

Y1 - 1991/8/30

N2 - The ability of diphenylhydantoin (DPH) to prevent hypoxia-induced [3H]glutamate release was examined in perfused rat hippocampal slices. Hypoxia (25 min; 95% N2/5% CO2) caused a prolonged release of [3H]glutamate, which was reduced significantly if DPH (20 μM) was present from the beginning of the perfusion. Perfusion with oxygenated medium (reoxygenation) following hypoxia also caused a pronounced release of glutamate. A therapeutic concentration of DPH, added before, during, or after hypoxia, decreased this release of glutamate. These results suggest that DPH may protect against glutamate-mediated neurotoxicity associated with stroke.

AB - The ability of diphenylhydantoin (DPH) to prevent hypoxia-induced [3H]glutamate release was examined in perfused rat hippocampal slices. Hypoxia (25 min; 95% N2/5% CO2) caused a prolonged release of [3H]glutamate, which was reduced significantly if DPH (20 μM) was present from the beginning of the perfusion. Perfusion with oxygenated medium (reoxygenation) following hypoxia also caused a pronounced release of glutamate. A therapeutic concentration of DPH, added before, during, or after hypoxia, decreased this release of glutamate. These results suggest that DPH may protect against glutamate-mediated neurotoxicity associated with stroke.

KW - Diphenylhydantoin

KW - Glutamate

KW - Hippocampus

KW - Hypoxia

KW - Ischemia

KW - Phenytoin

KW - Stroke

UR - http://www.scopus.com/inward/record.url?scp=0025820085&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025820085&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(91)90728-E

DO - 10.1016/0006-8993(91)90728-E

M3 - Article

C2 - 1682011

AN - SCOPUS:0025820085

SN - 0006-8993

VL - 558

SP - 127

EP - 130

JO - Brain research

JF - Brain research

IS - 1

ER -