Direct delivery and submicron patterning of DNA by nanofountain probe

Keun Ho Kim, Raymond Sanedrin, Seung W. Lee, Nicolaie Moldovan, Chad A. Mirkin, Horacio D. Espinosa*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

We present biomolecular patterning results using a nanofountain probe (NFP) that permits microfluidic direct delivery of oligonucleotides without hydrophilic modification of the tip for coating effectiveness. This is advantageous for integrated probes arrays that may require different functionalization to support writing with different inks, and in which damage of tips is more likely and critical. A linear array of NFPs was used to produce DNA nanoarrays. Routine feature sizes in the submicron range were obtained to prove both microfluidic transport and high-resolution writing. Gold nanoparticles functionalized with complementary sequence were hybridized to the patterned oligonucleotides, to demonstrate the biological activity of the deposited features. The results demonstrated the potential of our device as a production tool for nanofabrication of biomolecular arrays.

Original languageEnglish (US)
Title of host publicationProceedings of the SEM Annual Conference and Exposition on Experimental and Applied Mechanics 2007
Pages250-259
Number of pages10
StatePublished - 2007
EventSEM Annual Conference and Exposition on Experimental and Applied Mechanics 2007 - Springfield, MA, United States
Duration: Jun 3 2007Jun 6 2007

Publication series

NameProceedings of the SEM Annual Conference and Exposition on Experimental and Applied Mechanics 2007
Volume1

Other

OtherSEM Annual Conference and Exposition on Experimental and Applied Mechanics 2007
Country/TerritoryUnited States
CitySpringfield, MA
Period6/3/076/6/07

ASJC Scopus subject areas

  • Mechanical Engineering

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