Direct determination of the intracellular oxidation state of plutonium

Drew Gorman-Lewis, Baikuntha P. Aryal, Tatjana Paunesku, Stefan Vogt, Barry Lai, Gayle E. Woloschak, Mark P. Jensen*

*Corresponding author for this work

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Microprobe X-ray absorption near edge structure (μ-XANES) measurements were used to determine directly, for the first time, the oxidation state of intracellular plutonium in individual 0.1-μm 2 areas within single rat pheochromocytoma cells (PC12). The living cells were incubated in vitro for 3 h in the presence of Pu added to the media in different oxidation states (Pu(III), Pu(IV), and Pu(VI)) and in different chemical forms. Regardless of the initial oxidation state or chemical form of Pu presented to the cells, the XANES spectra of the intracellular Pu deposits were always consistent with tetravalent Pu even though the intracellular milieu is generally reducing.

Original languageEnglish (US)
Pages (from-to)7591-7597
Number of pages7
JournalInorganic chemistry
Volume50
Issue number16
DOIs
StatePublished - Aug 15 2011

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Inorganic Chemistry

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