Direct determination of the intracellular oxidation state of plutonium

Drew Gorman-Lewis; Baikuntha P. Aryal; Tatjana Paunesku; Stefan Vogt; Barry Lai; Gayle E. Woloschak; Mark P. Jensen

doi:10.1021/ic200588p

Direct determination of the intracellular oxidation state of plutonium

Drew Gorman-Lewis, Baikuntha P. Aryal, Tatjana Paunesku, Stefan Vogt, Barry Lai, Gayle E. Woloschak, Mark P. Jensen^*

^*Corresponding author for this work

Radiation Oncology

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Microprobe X-ray absorption near edge structure (μ-XANES) measurements were used to determine directly, for the first time, the oxidation state of intracellular plutonium in individual 0.1-μm ² areas within single rat pheochromocytoma cells (PC12). The living cells were incubated in vitro for 3 h in the presence of Pu added to the media in different oxidation states (Pu(III), Pu(IV), and Pu(VI)) and in different chemical forms. Regardless of the initial oxidation state or chemical form of Pu presented to the cells, the XANES spectra of the intracellular Pu deposits were always consistent with tetravalent Pu even though the intracellular milieu is generally reducing.

Original language	English (US)
Pages (from-to)	7591-7597
Number of pages	7
Journal	Inorganic chemistry
Volume	50
Issue number	16
DOIs	https://doi.org/10.1021/ic200588p
State	Published - Aug 15 2011

ASJC Scopus subject areas

Inorganic Chemistry
Physical and Theoretical Chemistry

Access to Document

10.1021/ic200588p

Cite this

@article{bdaf8e4130d3450f8c2d0c83c887ff41,

title = "Direct determination of the intracellular oxidation state of plutonium",

abstract = "Microprobe X-ray absorption near edge structure (μ-XANES) measurements were used to determine directly, for the first time, the oxidation state of intracellular plutonium in individual 0.1-μm 2 areas within single rat pheochromocytoma cells (PC12). The living cells were incubated in vitro for 3 h in the presence of Pu added to the media in different oxidation states (Pu(III), Pu(IV), and Pu(VI)) and in different chemical forms. Regardless of the initial oxidation state or chemical form of Pu presented to the cells, the XANES spectra of the intracellular Pu deposits were always consistent with tetravalent Pu even though the intracellular milieu is generally reducing.",

author = "Drew Gorman-Lewis and Aryal, {Baikuntha P.} and Tatjana Paunesku and Stefan Vogt and Barry Lai and Woloschak, {Gayle E.} and Jensen, {Mark P.}",

year = "2011",

month = aug,

day = "15",

doi = "10.1021/ic200588p",

language = "English (US)",

volume = "50",

pages = "7591--7597",

journal = "Inorganic Chemistry",

issn = "0020-1669",

publisher = "American Chemical Society",

number = "16",

}

TY - JOUR

T1 - Direct determination of the intracellular oxidation state of plutonium

AU - Gorman-Lewis, Drew

AU - Aryal, Baikuntha P.

AU - Paunesku, Tatjana

AU - Vogt, Stefan

AU - Lai, Barry

AU - Woloschak, Gayle E.

AU - Jensen, Mark P.

PY - 2011/8/15

Y1 - 2011/8/15

N2 - Microprobe X-ray absorption near edge structure (μ-XANES) measurements were used to determine directly, for the first time, the oxidation state of intracellular plutonium in individual 0.1-μm 2 areas within single rat pheochromocytoma cells (PC12). The living cells were incubated in vitro for 3 h in the presence of Pu added to the media in different oxidation states (Pu(III), Pu(IV), and Pu(VI)) and in different chemical forms. Regardless of the initial oxidation state or chemical form of Pu presented to the cells, the XANES spectra of the intracellular Pu deposits were always consistent with tetravalent Pu even though the intracellular milieu is generally reducing.

AB - Microprobe X-ray absorption near edge structure (μ-XANES) measurements were used to determine directly, for the first time, the oxidation state of intracellular plutonium in individual 0.1-μm 2 areas within single rat pheochromocytoma cells (PC12). The living cells were incubated in vitro for 3 h in the presence of Pu added to the media in different oxidation states (Pu(III), Pu(IV), and Pu(VI)) and in different chemical forms. Regardless of the initial oxidation state or chemical form of Pu presented to the cells, the XANES spectra of the intracellular Pu deposits were always consistent with tetravalent Pu even though the intracellular milieu is generally reducing.

UR - http://www.scopus.com/inward/record.url?scp=80051534555&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80051534555&partnerID=8YFLogxK

U2 - 10.1021/ic200588p

DO - 10.1021/ic200588p

M3 - Article

C2 - 21755934

AN - SCOPUS:80051534555

SN - 0020-1669

VL - 50

SP - 7591

EP - 7597

JO - Inorganic Chemistry

JF - Inorganic Chemistry

IS - 16

ER -

Direct determination of the intracellular oxidation state of plutonium

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this