Direct genetic analysis of ten cancer cells: Tuning sensor structure and molecular probe design for efficient mRNA capture

Elizaveta Vasilyeva; Brian Lam; Zhichao Fang; Mark D. Minden; Edward H. Sargent; Shana O. Kelley

doi:10.1002/anie.201006793

Direct genetic analysis of ten cancer cells: Tuning sensor structure and molecular probe design for efficient mRNA capture

Elizaveta Vasilyeva, Brian Lam, Zhichao Fang, Mark D. Minden, Edward H. Sargent, Shana O. Kelley

Chemistry

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

A chip-based platform is reported that is able to detect as few as 10 cancer cells. By developing sub-milliscale sensors that are able to capture slow moving biological targets with high efficiency (see picture; scale bar 50 μm), cancer-specific sequences were detected in crude lysates of leukemia cells. This achievement relied on the development of a new type of molecular probe that improves the solubility and performance of neutral nucleic acids.

Original language	English (US)
Pages (from-to)	4137-4141
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	50
Issue number	18
DOIs	https://doi.org/10.1002/anie.201006793
State	Published - Apr 26 2011

Keywords

biosensor
microchips
microelectrodes
nucleic acids

ASJC Scopus subject areas

General Chemistry
Catalysis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/anie.201006793

Cite this

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abstract = "A chip-based platform is reported that is able to detect as few as 10 cancer cells. By developing sub-milliscale sensors that are able to capture slow moving biological targets with high efficiency (see picture; scale bar 50 μm), cancer-specific sequences were detected in crude lysates of leukemia cells. This achievement relied on the development of a new type of molecular probe that improves the solubility and performance of neutral nucleic acids.",

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AU - Fang, Zhichao

AU - Minden, Mark D.

AU - Sargent, Edward H.

AU - Kelley, Shana O.

PY - 2011/4/26

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AB - A chip-based platform is reported that is able to detect as few as 10 cancer cells. By developing sub-milliscale sensors that are able to capture slow moving biological targets with high efficiency (see picture; scale bar 50 μm), cancer-specific sequences were detected in crude lysates of leukemia cells. This achievement relied on the development of a new type of molecular probe that improves the solubility and performance of neutral nucleic acids.

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KW - microelectrodes

KW - nucleic acids

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Direct genetic analysis of ten cancer cells: Tuning sensor structure and molecular probe design for efficient mRNA capture

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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