Direct mecA detection from blood culture bottles by branched-DNA signal amplification

X. Zheng, C. P. Kolbert, P. Varga-Delmore, J. Arruda, M. Lewis, J. Kolberg, F. R. Cockerill, D. H. Persing*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

A branched-DNA (bDNA) signal amplification method was used to detect the meca gene directly from blood culture broth growing staphylococci. BACTEC blood culture bottles with positive growth indices and containing staphylococcus-like organisms as shown by Gram stain were tested for the presence of the meca gene. Comparison of test results was done among 225 patients (one blood culture from each patient). Compared with PCR, the sensitivity and specificity of the bDNA method are 100 and 99%, respectively. The bDNA test is carried out in a 96-well format and requires approximately 6 h to perform. Our preliminary results suggest that direct detection of the meca gene by bDNA signal amplification is (i) sensitive enough to detect meca directly from blood culture bottles without the requirement for subculture and (ii) as sensitive and specific as the PCR-based method.

Original languageEnglish (US)
Pages (from-to)4192-4193
Number of pages2
JournalJournal of clinical microbiology
Volume37
Issue number12
DOIs
StatePublished - Dec 1999

ASJC Scopus subject areas

  • Microbiology (medical)

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