Direct observation of nanoparticle-cancer cell nucleus interactions

Duncan Hieu M Dam; Jung Heon Lee; Patrick N. Sisco; Dick T. Co; Ming Zhang; Michael R. Wasielewski; Teri W. Odom

doi:10.1021/nn300296p

Direct observation of nanoparticle-cancer cell nucleus interactions

Duncan Hieu M Dam, Jung Heon Lee, Patrick N. Sisco, Dick T. Co, Ming Zhang, Michael R. Wasielewski, Teri W. Odom^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

251 Scopus citations

Abstract

We report the direct visualization of interactions between drug-loaded nanoparticles and the cancer cell nucleus. Nanoconstructs composed of nucleolin-specific aptamers and gold nanostars were actively transported to the nucleus and induced major changes to the nuclear phenotype via nuclear envelope invaginations near the site of the construct. The number of local deformations could be increased by ultrafast, light-triggered release of the aptamers from the surface of the gold nanostars. Cancer cells with more nuclear envelope folding showed increased caspase 3 and 7 activity (apoptosis) as well as decreased cell viability. This newly revealed correlation between drug-induced changes in nuclear phenotype and increased therapeutic efficacy could provide new insight for nuclear-targeted cancer therapy.

Original language	English (US)
Pages (from-to)	3318-3326
Number of pages	9
Journal	ACS nano
Volume	6
Issue number	4
DOIs	https://doi.org/10.1021/nn300296p
State	Published - Apr 24 2012

Funding

Keywords

aptamers
drug delivery
gold nanoparticles
light-triggered release
nanoparticle-nucleus interactions
nucleolin

ASJC Scopus subject areas

General Engineering
General Materials Science
General Physics and Astronomy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/nn300296p

Cite this

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title = "Direct observation of nanoparticle-cancer cell nucleus interactions",

abstract = "We report the direct visualization of interactions between drug-loaded nanoparticles and the cancer cell nucleus. Nanoconstructs composed of nucleolin-specific aptamers and gold nanostars were actively transported to the nucleus and induced major changes to the nuclear phenotype via nuclear envelope invaginations near the site of the construct. The number of local deformations could be increased by ultrafast, light-triggered release of the aptamers from the surface of the gold nanostars. Cancer cells with more nuclear envelope folding showed increased caspase 3 and 7 activity (apoptosis) as well as decreased cell viability. This newly revealed correlation between drug-induced changes in nuclear phenotype and increased therapeutic efficacy could provide new insight for nuclear-targeted cancer therapy.",

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AU - Dam, Duncan Hieu M

AU - Lee, Jung Heon

AU - Sisco, Patrick N.

AU - Co, Dick T.

AU - Zhang, Ming

AU - Wasielewski, Michael R.

AU - Odom, Teri W.

PY - 2012/4/24

Y1 - 2012/4/24

N2 - We report the direct visualization of interactions between drug-loaded nanoparticles and the cancer cell nucleus. Nanoconstructs composed of nucleolin-specific aptamers and gold nanostars were actively transported to the nucleus and induced major changes to the nuclear phenotype via nuclear envelope invaginations near the site of the construct. The number of local deformations could be increased by ultrafast, light-triggered release of the aptamers from the surface of the gold nanostars. Cancer cells with more nuclear envelope folding showed increased caspase 3 and 7 activity (apoptosis) as well as decreased cell viability. This newly revealed correlation between drug-induced changes in nuclear phenotype and increased therapeutic efficacy could provide new insight for nuclear-targeted cancer therapy.

AB - We report the direct visualization of interactions between drug-loaded nanoparticles and the cancer cell nucleus. Nanoconstructs composed of nucleolin-specific aptamers and gold nanostars were actively transported to the nucleus and induced major changes to the nuclear phenotype via nuclear envelope invaginations near the site of the construct. The number of local deformations could be increased by ultrafast, light-triggered release of the aptamers from the surface of the gold nanostars. Cancer cells with more nuclear envelope folding showed increased caspase 3 and 7 activity (apoptosis) as well as decreased cell viability. This newly revealed correlation between drug-induced changes in nuclear phenotype and increased therapeutic efficacy could provide new insight for nuclear-targeted cancer therapy.

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KW - drug delivery

KW - gold nanoparticles

KW - light-triggered release

KW - nanoparticle-nucleus interactions

KW - nucleolin

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Direct observation of nanoparticle-cancer cell nucleus interactions

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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