Direct radioimmunoassay of progesterone in saliva

Yu Cai Lu, Robert T. Chatterton, Kirsten M. Vogelsong, Lynette K. May

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


We have developed a simple, direct radioimmunoassay for progesterone in saliva. The correlation coefficient (r) between the direct assay and an extraction procedure was 0.92 (n=65, P<0.001), and the correlation between concurrent serum and salivary progesterone concentrations in the luteal phases of menstrual cycles of 48 women was 0.75 (P<0.001). Whereas certain polystyrene and polyethylene vials and tubes were found to bind and remove up to 87% of the progesterone from saliva, other plastic and glass surfaces were satisfactory for the procedure. Intraassay and interassay CVs from values greater than 300 pmol/L were 12.0 and 12.4%, respectively. The assay sensitivity was 48 pmol/L. Collection of saliva is a more convenient and less invasive technique for frequent sample collection than phlebotomy, and is useful for monitoring ovulation and assessment of luteal function in women clinically.

Original languageEnglish (US)
Pages (from-to)149-163
Number of pages15
JournalJournal of Immunoassay
Issue number2
StatePublished - Jan 1 1997


  • RIA
  • human
  • menstrual cycle
  • progesterone
  • saliva

ASJC Scopus subject areas

  • Immunology
  • Pharmacology


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