Direct transformation of Neisseria gonorrhoeae by gel-isolated DNA

Kristen Hoikka Pritchard, H. Steven Seifert*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The naturally competent organism, Neisseria gonorrhoeae, can efficiently transform a marker carried on DNA purified in low-melting-temperature agarose without prior purification or dilution. Neither the agarose or buffer components inhibit transformation frequencies, but exposure to UV irradiation completely abrogates transformation.

Original languageEnglish (US)
Pages (from-to)315-317
Number of pages3
JournalMolecular Biotechnology
Volume4
Issue number3
DOIs
StatePublished - Dec 1995

Keywords

  • DNA transformation
  • Neisseria gonorrhoeae
  • UV irradiation
  • ethidium bromide
  • low-melting agarose

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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