Direct transformation of Neisseria gonorrhoeae by gel-isolated DNA

Kristen Hoikka Pritchard; H. Steven Seifert

doi:10.1007/BF02779023

Direct transformation of Neisseria gonorrhoeae by gel-isolated DNA

Kristen Hoikka Pritchard, H. Steven Seifert^*

^*Corresponding author for this work

Microbiology-Immunology

Research output: Contribution to journal › Article › peer-review

Abstract

The naturally competent organism, Neisseria gonorrhoeae, can efficiently transform a marker carried on DNA purified in low-melting-temperature agarose without prior purification or dilution. Neither the agarose or buffer components inhibit transformation frequencies, but exposure to UV irradiation completely abrogates transformation.

Original language	English (US)
Pages (from-to)	315-317
Number of pages	3
Journal	Molecular Biotechnology
Volume	4
Issue number	3
DOIs	https://doi.org/10.1007/BF02779023
State	Published - Dec 1995

Keywords

DNA transformation
Neisseria gonorrhoeae
UV irradiation
ethidium bromide
low-melting agarose

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Bioengineering
Molecular Biology
Biochemistry
Biotechnology

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10.1007/BF02779023

Cite this

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title = "Direct transformation of Neisseria gonorrhoeae by gel-isolated DNA",

abstract = "The naturally competent organism, Neisseria gonorrhoeae, can efficiently transform a marker carried on DNA purified in low-melting-temperature agarose without prior purification or dilution. Neither the agarose or buffer components inhibit transformation frequencies, but exposure to UV irradiation completely abrogates transformation.",

keywords = "DNA transformation, Neisseria gonorrhoeae, UV irradiation, ethidium bromide, low-melting agarose",

author = "Pritchard, {Kristen Hoikka} and Seifert, {H. Steven}",

year = "1995",

month = dec,

doi = "10.1007/BF02779023",

language = "English (US)",

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pages = "315--317",

journal = "Molecular Biotechnology",

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T1 - Direct transformation of Neisseria gonorrhoeae by gel-isolated DNA

AU - Pritchard, Kristen Hoikka

AU - Seifert, H. Steven

PY - 1995/12

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N2 - The naturally competent organism, Neisseria gonorrhoeae, can efficiently transform a marker carried on DNA purified in low-melting-temperature agarose without prior purification or dilution. Neither the agarose or buffer components inhibit transformation frequencies, but exposure to UV irradiation completely abrogates transformation.

AB - The naturally competent organism, Neisseria gonorrhoeae, can efficiently transform a marker carried on DNA purified in low-melting-temperature agarose without prior purification or dilution. Neither the agarose or buffer components inhibit transformation frequencies, but exposure to UV irradiation completely abrogates transformation.

KW - DNA transformation

KW - Neisseria gonorrhoeae

KW - UV irradiation

KW - ethidium bromide

KW - low-melting agarose

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Direct transformation of Neisseria gonorrhoeae by gel-isolated DNA

Abstract

Keywords

ASJC Scopus subject areas

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