Disassembly of the nuclear envelope of spisula oocytes in a cell-free system

George Dessev*, Robert Palazzo, Lionel Rebhun, Robert Goldman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


Nuclei isolated from oocytes of the surf clam Spisula solidissima are disassembled when exposed to extracts from maturing oocytes. In the course of this process the nuclear lamina undergoes a marked reduction in size and the nuclear membrane appears to be fragmented into vesicles. These events are accompanied by extensive phosphorylation of the oocyte 67-kDa lamin and its solubilization. The changes observed are similar to those which occur in vivo in activated Spisula oocytes. Nuclear envelope breakdown in vitro requires ATP and Mg2+, but not Ca2+. It is not affected by protease inhibitors and is inhibited by alkaline phosphatase.

Original languageEnglish (US)
Pages (from-to)496-504
Number of pages9
JournalDevelopmental Biology
Issue number2
StatePublished - Feb 1989

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology


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