Discoidin I-membrane interactions III. Interaction of discoidin I with living Dictyostelium discoideum cells

James R. Bartles, Barbara C. Santoro, William A. Frazier*

*Corresponding author for this work

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We have examined the interaction of exogenous 125I-discoidin I with living wild type (NC-4) Dictyostelium discoideum cells and the subcellular localization of endogenous discoidin I during development. The living cells bind 125I-discoidin I relatively rapidly, with an apparent steady state being reached within 40 min at 23°C. The rate of association is reduced to a similar extent by lowering the temperature to 4°C and by glutaraldehyde fixation of the cells. The level of steady-state binding is not developmentally regulated. Living cells do not degrade significant amounts of the 125I-discoidin I during incubations at 23°C. Differentiated cells bind the 125I-discoidin I via both the carbohydrate or C site receptors and the ionic or I site receptors, first identified on the corresponding glutaraldehyde-fixed cells (Bartles, J.R. and Frazier, W.A. (1982) Biochim. Biophys. Acta 687, 121-128). Depending on the concentration of 125I-discoidin I, 50% or more of the binding to the 8.5-h differentiated cells is via the I sites. Binding exhibits apparent positive cooperativity with respect to discoidin I. This causes the binding to appear nonsaturable, with a capacity for more than 107 discoidin I tetramers per cell. Independent verification of the nonsaturability of the interaction was obtained from experiments using diazotized [125I]iodosulfanilic acid to radiolabel the surfaces of cells treated with increasing concentrations of unlabeled discoidin I. The susceptibility of the surface radiolabeled discoidin I to various extractive treatments of the cellular particulate fraction suggests that as much as 10% is very tightly associated with cellular membranes. Dissociation experiments also suggest the existence of an irreversible component of binding, the magnitude of which increases with time of association. While the 8.5-h differentiated NC-4 cells exhibit maximal developmental cohesiveness and contain about 5·106 discoidin I tetramers per cell, cell surface labeling and radioimmunoassay indicate that they display only 1.3·103 tetramers per cell on their surface and only 6·103 tetramers per cell in their surrounding extracellular medium.

Original languageEnglish (US)
Pages (from-to)137-146
Number of pages10
JournalBBA - Biomembranes
Volume687
Issue number2
DOIs
StatePublished - May 7 1982

Fingerprint

Dictyostelium
Membranes
Glutaral
Cells
Association reactions
Labeling
Experiments
Carbohydrates
Acids
Discoidins
Temperature
Radioimmunoassay

Keywords

  • (D. discoideum)
  • Binding cooperativity
  • Discoidin I-membrane interaction

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

@article{720a5e349a36457aab1e6db73aabba97,
title = "Discoidin I-membrane interactions III. Interaction of discoidin I with living Dictyostelium discoideum cells",
abstract = "We have examined the interaction of exogenous 125I-discoidin I with living wild type (NC-4) Dictyostelium discoideum cells and the subcellular localization of endogenous discoidin I during development. The living cells bind 125I-discoidin I relatively rapidly, with an apparent steady state being reached within 40 min at 23°C. The rate of association is reduced to a similar extent by lowering the temperature to 4°C and by glutaraldehyde fixation of the cells. The level of steady-state binding is not developmentally regulated. Living cells do not degrade significant amounts of the 125I-discoidin I during incubations at 23°C. Differentiated cells bind the 125I-discoidin I via both the carbohydrate or C site receptors and the ionic or I site receptors, first identified on the corresponding glutaraldehyde-fixed cells (Bartles, J.R. and Frazier, W.A. (1982) Biochim. Biophys. Acta 687, 121-128). Depending on the concentration of 125I-discoidin I, 50{\%} or more of the binding to the 8.5-h differentiated cells is via the I sites. Binding exhibits apparent positive cooperativity with respect to discoidin I. This causes the binding to appear nonsaturable, with a capacity for more than 107 discoidin I tetramers per cell. Independent verification of the nonsaturability of the interaction was obtained from experiments using diazotized [125I]iodosulfanilic acid to radiolabel the surfaces of cells treated with increasing concentrations of unlabeled discoidin I. The susceptibility of the surface radiolabeled discoidin I to various extractive treatments of the cellular particulate fraction suggests that as much as 10{\%} is very tightly associated with cellular membranes. Dissociation experiments also suggest the existence of an irreversible component of binding, the magnitude of which increases with time of association. While the 8.5-h differentiated NC-4 cells exhibit maximal developmental cohesiveness and contain about 5·106 discoidin I tetramers per cell, cell surface labeling and radioimmunoassay indicate that they display only 1.3·103 tetramers per cell on their surface and only 6·103 tetramers per cell in their surrounding extracellular medium.",
keywords = "(D. discoideum), Binding cooperativity, Discoidin I-membrane interaction",
author = "Bartles, {James R.} and Santoro, {Barbara C.} and Frazier, {William A.}",
year = "1982",
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volume = "687",
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Discoidin I-membrane interactions III. Interaction of discoidin I with living Dictyostelium discoideum cells. / Bartles, James R.; Santoro, Barbara C.; Frazier, William A.

In: BBA - Biomembranes, Vol. 687, No. 2, 07.05.1982, p. 137-146.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Discoidin I-membrane interactions III. Interaction of discoidin I with living Dictyostelium discoideum cells

AU - Bartles, James R.

AU - Santoro, Barbara C.

AU - Frazier, William A.

PY - 1982/5/7

Y1 - 1982/5/7

N2 - We have examined the interaction of exogenous 125I-discoidin I with living wild type (NC-4) Dictyostelium discoideum cells and the subcellular localization of endogenous discoidin I during development. The living cells bind 125I-discoidin I relatively rapidly, with an apparent steady state being reached within 40 min at 23°C. The rate of association is reduced to a similar extent by lowering the temperature to 4°C and by glutaraldehyde fixation of the cells. The level of steady-state binding is not developmentally regulated. Living cells do not degrade significant amounts of the 125I-discoidin I during incubations at 23°C. Differentiated cells bind the 125I-discoidin I via both the carbohydrate or C site receptors and the ionic or I site receptors, first identified on the corresponding glutaraldehyde-fixed cells (Bartles, J.R. and Frazier, W.A. (1982) Biochim. Biophys. Acta 687, 121-128). Depending on the concentration of 125I-discoidin I, 50% or more of the binding to the 8.5-h differentiated cells is via the I sites. Binding exhibits apparent positive cooperativity with respect to discoidin I. This causes the binding to appear nonsaturable, with a capacity for more than 107 discoidin I tetramers per cell. Independent verification of the nonsaturability of the interaction was obtained from experiments using diazotized [125I]iodosulfanilic acid to radiolabel the surfaces of cells treated with increasing concentrations of unlabeled discoidin I. The susceptibility of the surface radiolabeled discoidin I to various extractive treatments of the cellular particulate fraction suggests that as much as 10% is very tightly associated with cellular membranes. Dissociation experiments also suggest the existence of an irreversible component of binding, the magnitude of which increases with time of association. While the 8.5-h differentiated NC-4 cells exhibit maximal developmental cohesiveness and contain about 5·106 discoidin I tetramers per cell, cell surface labeling and radioimmunoassay indicate that they display only 1.3·103 tetramers per cell on their surface and only 6·103 tetramers per cell in their surrounding extracellular medium.

AB - We have examined the interaction of exogenous 125I-discoidin I with living wild type (NC-4) Dictyostelium discoideum cells and the subcellular localization of endogenous discoidin I during development. The living cells bind 125I-discoidin I relatively rapidly, with an apparent steady state being reached within 40 min at 23°C. The rate of association is reduced to a similar extent by lowering the temperature to 4°C and by glutaraldehyde fixation of the cells. The level of steady-state binding is not developmentally regulated. Living cells do not degrade significant amounts of the 125I-discoidin I during incubations at 23°C. Differentiated cells bind the 125I-discoidin I via both the carbohydrate or C site receptors and the ionic or I site receptors, first identified on the corresponding glutaraldehyde-fixed cells (Bartles, J.R. and Frazier, W.A. (1982) Biochim. Biophys. Acta 687, 121-128). Depending on the concentration of 125I-discoidin I, 50% or more of the binding to the 8.5-h differentiated cells is via the I sites. Binding exhibits apparent positive cooperativity with respect to discoidin I. This causes the binding to appear nonsaturable, with a capacity for more than 107 discoidin I tetramers per cell. Independent verification of the nonsaturability of the interaction was obtained from experiments using diazotized [125I]iodosulfanilic acid to radiolabel the surfaces of cells treated with increasing concentrations of unlabeled discoidin I. The susceptibility of the surface radiolabeled discoidin I to various extractive treatments of the cellular particulate fraction suggests that as much as 10% is very tightly associated with cellular membranes. Dissociation experiments also suggest the existence of an irreversible component of binding, the magnitude of which increases with time of association. While the 8.5-h differentiated NC-4 cells exhibit maximal developmental cohesiveness and contain about 5·106 discoidin I tetramers per cell, cell surface labeling and radioimmunoassay indicate that they display only 1.3·103 tetramers per cell on their surface and only 6·103 tetramers per cell in their surrounding extracellular medium.

KW - (D. discoideum)

KW - Binding cooperativity

KW - Discoidin I-membrane interaction

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