Discrimination of fluorinated uridine metabolites in N‐417 small cell lung cancer cell extracts via 19F‐ and 31P‐NMR

Nathanael V. Pederson; James A. Zanghi; William M. Miller; Richard H. Knop

doi:10.1002/mrm.1910310217

Discrimination of fluorinated uridine metabolites in N‐417 small cell lung cancer cell extracts via ¹⁹F‐ and ³¹P‐NMR

Nathanael V. Pederson, James A. Zanghi, William M. Miller, Richard H. Knop^*

^*Corresponding author for this work

Research output: Contribution to journal › Letter › peer-review

9 Scopus citations

Abstract

³¹P‐NMR extract spectra of N‐417 Small Cell Lung Cancer (SCLC) cells cultured with fluorouridine (FUrd) reveal new peaks with chemical shifts in the diphosphodiester and nucleoside triphosphate regions. These peaks were identified as FUTP, FUDP, FUDP‐glucose, FUDP‐glucuronate, FUDP‐GlcNAc, and FUDP‐GalNAc via enzymatic conversion and ¹⁹F‐and ³¹P‐NYR analysis. Distinct ¹⁹F chemical shifts were assigned for FUTP, FUDP, and the FUDP‐sugars.

Original language	English (US)
Pages (from-to)	224-228
Number of pages	5
Journal	Magnetic resonance in medicine
Volume	31
Issue number	2
DOIs	https://doi.org/10.1002/mrm.1910310217
State	Published - Feb 1994
Externally published	Yes

Keywords

NMR
diphosphodiester
fluorouridine
small cell lung cancer

ASJC Scopus subject areas

Radiology Nuclear Medicine and imaging

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1002/mrm.1910310217

Cite this

@article{0ab4d5d37cbb4be5bcb15f93068c8e9f,

title = "Discrimination of fluorinated uridine metabolites in N‐417 small cell lung cancer cell extracts via 19F‐ and 31P‐NMR",

abstract = "31P‐NMR extract spectra of N‐417 Small Cell Lung Cancer (SCLC) cells cultured with fluorouridine (FUrd) reveal new peaks with chemical shifts in the diphosphodiester and nucleoside triphosphate regions. These peaks were identified as FUTP, FUDP, FUDP‐glucose, FUDP‐glucuronate, FUDP‐GlcNAc, and FUDP‐GalNAc via enzymatic conversion and 19F‐and 31P‐NYR analysis. Distinct 19F chemical shifts were assigned for FUTP, FUDP, and the FUDP‐sugars.",

keywords = "NMR, diphosphodiester, fluorouridine, small cell lung cancer",

author = "Pederson, {Nathanael V.} and Zanghi, {James A.} and Miller, {William M.} and Knop, {Richard H.}",

year = "1994",

month = feb,

doi = "10.1002/mrm.1910310217",

language = "English (US)",

volume = "31",

pages = "224--228",

journal = "Magnetic resonance in medicine",

issn = "0740-3194",

publisher = "John Wiley and Sons Inc.",

number = "2",

}

TY - JOUR

T1 - Discrimination of fluorinated uridine metabolites in N‐417 small cell lung cancer cell extracts via 19F‐ and 31P‐NMR

AU - Pederson, Nathanael V.

AU - Zanghi, James A.

AU - Miller, William M.

AU - Knop, Richard H.

PY - 1994/2

Y1 - 1994/2

N2 - 31P‐NMR extract spectra of N‐417 Small Cell Lung Cancer (SCLC) cells cultured with fluorouridine (FUrd) reveal new peaks with chemical shifts in the diphosphodiester and nucleoside triphosphate regions. These peaks were identified as FUTP, FUDP, FUDP‐glucose, FUDP‐glucuronate, FUDP‐GlcNAc, and FUDP‐GalNAc via enzymatic conversion and 19F‐and 31P‐NYR analysis. Distinct 19F chemical shifts were assigned for FUTP, FUDP, and the FUDP‐sugars.

AB - 31P‐NMR extract spectra of N‐417 Small Cell Lung Cancer (SCLC) cells cultured with fluorouridine (FUrd) reveal new peaks with chemical shifts in the diphosphodiester and nucleoside triphosphate regions. These peaks were identified as FUTP, FUDP, FUDP‐glucose, FUDP‐glucuronate, FUDP‐GlcNAc, and FUDP‐GalNAc via enzymatic conversion and 19F‐and 31P‐NYR analysis. Distinct 19F chemical shifts were assigned for FUTP, FUDP, and the FUDP‐sugars.

KW - NMR

KW - diphosphodiester

KW - fluorouridine

KW - small cell lung cancer

UR - http://www.scopus.com/inward/record.url?scp=0027954656&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027954656&partnerID=8YFLogxK

U2 - 10.1002/mrm.1910310217

DO - 10.1002/mrm.1910310217

M3 - Letter

C2 - 8133759

AN - SCOPUS:0027954656

SN - 0740-3194

VL - 31

SP - 224

EP - 228

JO - Magnetic resonance in medicine

JF - Magnetic resonance in medicine

IS - 2

ER -