Discrimination of fluorinated uridine metabolites in N‐417 small cell lung cancer cell extracts via 19F‐ and 31P‐NMR

Nathanael V. Pederson, James A. Zanghi, William M Miller, Richard H. Knop*

*Corresponding author for this work

Research output: Contribution to journalLetter

7 Citations (Scopus)

Abstract

31P‐NMR extract spectra of N‐417 Small Cell Lung Cancer (SCLC) cells cultured with fluorouridine (FUrd) reveal new peaks with chemical shifts in the diphosphodiester and nucleoside triphosphate regions. These peaks were identified as FUTP, FUDP, FUDP‐glucose, FUDP‐glucuronate, FUDP‐GlcNAc, and FUDP‐GalNAc via enzymatic conversion and 19F‐and 31P‐NYR analysis. Distinct 19F chemical shifts were assigned for FUTP, FUDP, and the FUDP‐sugars.

Original languageEnglish (US)
Pages (from-to)224-228
Number of pages5
JournalMagnetic resonance in medicine
Volume31
Issue number2
DOIs
StatePublished - Jan 1 1994

Fingerprint

Uridine
Small Cell Lung Carcinoma
Cell Extracts
Nucleosides
Cultured Cells
5-fluorouridine 5'-triphosphate
triphosphoric acid

Keywords

  • NMR
  • diphosphodiester
  • fluorouridine
  • small cell lung cancer

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

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title = "Discrimination of fluorinated uridine metabolites in N‐417 small cell lung cancer cell extracts via 19F‐ and 31P‐NMR",
abstract = "31P‐NMR extract spectra of N‐417 Small Cell Lung Cancer (SCLC) cells cultured with fluorouridine (FUrd) reveal new peaks with chemical shifts in the diphosphodiester and nucleoside triphosphate regions. These peaks were identified as FUTP, FUDP, FUDP‐glucose, FUDP‐glucuronate, FUDP‐GlcNAc, and FUDP‐GalNAc via enzymatic conversion and 19F‐and 31P‐NYR analysis. Distinct 19F chemical shifts were assigned for FUTP, FUDP, and the FUDP‐sugars.",
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}

Discrimination of fluorinated uridine metabolites in N‐417 small cell lung cancer cell extracts via 19F‐ and 31P‐NMR. / Pederson, Nathanael V.; Zanghi, James A.; Miller, William M; Knop, Richard H.

In: Magnetic resonance in medicine, Vol. 31, No. 2, 01.01.1994, p. 224-228.

Research output: Contribution to journalLetter

TY - JOUR

T1 - Discrimination of fluorinated uridine metabolites in N‐417 small cell lung cancer cell extracts via 19F‐ and 31P‐NMR

AU - Pederson, Nathanael V.

AU - Zanghi, James A.

AU - Miller, William M

AU - Knop, Richard H.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - 31P‐NMR extract spectra of N‐417 Small Cell Lung Cancer (SCLC) cells cultured with fluorouridine (FUrd) reveal new peaks with chemical shifts in the diphosphodiester and nucleoside triphosphate regions. These peaks were identified as FUTP, FUDP, FUDP‐glucose, FUDP‐glucuronate, FUDP‐GlcNAc, and FUDP‐GalNAc via enzymatic conversion and 19F‐and 31P‐NYR analysis. Distinct 19F chemical shifts were assigned for FUTP, FUDP, and the FUDP‐sugars.

AB - 31P‐NMR extract spectra of N‐417 Small Cell Lung Cancer (SCLC) cells cultured with fluorouridine (FUrd) reveal new peaks with chemical shifts in the diphosphodiester and nucleoside triphosphate regions. These peaks were identified as FUTP, FUDP, FUDP‐glucose, FUDP‐glucuronate, FUDP‐GlcNAc, and FUDP‐GalNAc via enzymatic conversion and 19F‐and 31P‐NYR analysis. Distinct 19F chemical shifts were assigned for FUTP, FUDP, and the FUDP‐sugars.

KW - NMR

KW - diphosphodiester

KW - fluorouridine

KW - small cell lung cancer

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U2 - 10.1002/mrm.1910310217

DO - 10.1002/mrm.1910310217

M3 - Letter

VL - 31

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EP - 228

JO - Magnetic Resonance in Medicine

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SN - 0740-3194

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