Discrimination of ischemic and hemorrhagic strokes using a multiplexed, mass spectrometry-based assay for serum apolipoproteins coupled to multi-marker ROC algorithm

Mary F. Lopez*, David A. Sarracino, Amol Prakash, Michael Athanas, Bryan Krastins, Taha Rezai, Jennifer N. Sutton, Scott Peterman, Oksana Gvozdyak, Sherry Chou, Eng Lo, Ferdinand Buonanno, Mingming Ning

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Purpose: Typically, apolipoproteins are individually measured in blood by immunoassay. In this report, we describe the development of a multiplexed selected reaction monitoring (SRM) based assay for a panel of apolipoproteins and its application to a clinical cohort of samples derived from acute stroke patients. Experimental Design: An SRM assay for a panel of nine apolipoproteins was developed on a triple quadrupole mass spectrometer. Quantitative data for each apolipoprotein were analyzed to determine expression ratio and receiver operating characteristic (ROC) values for ischemic versus hemorrhagic stroke. Results: The optimized SRM assay was used to interrogate a small cohort of well-characterized plasma samples obtained from patients with acute ischemic and hemorrhagic strokes. The ROC analyses demonstrated good classification power for several single apolipoproteins, most notably apoC-III and apoC-I. When a novel multi-marker ROC algorithm was applied, the ischemic versus hemorrhagic groups were best differentiated by a combination of apoC-III and apoA-I with an area under the curve (AUC) value of 0.92. Conclusions and clinical relevance: This proof-of-concept study provides interesting and provocative data for distinguishing ischemic versus hemorrhage within first week of symptom onset. However, the observations are based on one cohort of patient samples and further confirmation will be required.

Original languageEnglish (US)
Pages (from-to)190-200
Number of pages11
JournalProteomics - Clinical Applications
Volume6
Issue number3-4
DOIs
StatePublished - Apr 2012
Externally publishedYes

Keywords

  • Biomarker
  • Cerebrovascular
  • Hemorrhagic
  • Ischemic
  • Mass spectrometry

ASJC Scopus subject areas

  • Clinical Biochemistry

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