Dispase/triton treated acellular dermal matrix as a dermal substitute in rats

Robert J. Walter*, Lawrence J. Jennings, Takayoshi Matsuda, Hernan M. Reyes, Marella Hanumadass

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Acellular allogeneic dermal matrix (ADM) was prepared using Dispase and Triton X-100 detergent, and its effectiveness as a dermal substitute is described. Onlay split thickness skin autografts (STSG; 0.015-inch thick) placed onto ADM showed poor survival when the skin was grafted simultaneously with the ADM but good survival when STSG placement was delayed more than 1 week after ADM implantation. For one-step grafting, thinner ADMs and STSGs were required to obtain good graft survival. The use of 0.012- or 0.015-inch thick ADM in conjunction with simultaneously applied, ultra-thin (0.005-inch thick) STSG resulted in good graft survival (89-5% of graft area) and reduced wound contracture. Dispase/Triton treated ADM derived from animal or human skin may be useful clinically in full-thickness skin defects, providing a pliant yet lasting dermal bed for subsequent or immediate skin grafting.

Original languageEnglish (US)
Pages (from-to)371-374
Number of pages4
JournalCurrent Surgery
Volume54
Issue number6
StatePublished - Jul 1 1997

ASJC Scopus subject areas

  • Surgery

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