Disruption of ATP-binding cassette B8 in mice leads to cardiomyopathy through a decrease in mitochondrial iron export

Yoshihiko Ichikawa, Marina Bayeva, Mohsen Ghanefar, Vishnu Potini, Lin Sun, R Kannan Mutharasan, Rongxue Wu, Arineh Khechaduri, Tejaswitha Jairaj Naik, Hossein Ardehali*

*Corresponding author for this work

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Mitochondrial iron levels are tightly regulated, as iron is essential for the synthesis of Fe/S clusters and heme in the mitochondria, but high levels can cause oxidative stress. The ATP-binding cassette (ABC) transporter ABCB8 is a mitochondrial inner membrane protein with an unknown function. Here, we show that ABCB8 is involved in mitochondrial iron export and is essential for baseline cardiac function. Induced genetic deletion of ABCB8 in mouse hearts resulted in mitochondrial iron accumulation and cardiomyopathy, as assessed by echocardiography and invasive hemodynamics. Mice with ABCB8 deletion in the heart also displayed mitochondrial damage, and higher levels of reactive oxygen species and cell death. Down-regulation of ABCB8 in vitro resulted in decreased iron export from isolated mitochondria, whereas its overexpression had the opposite effect. Furthermore, ABCB8 is needed for the maturation of the cytosolic Fe/S proteins, as its deletion in vitro and in vivo led to decreased activity of cytosolic, but not mitochondrial, iron-sulfur-containing enzymes. These results indicate that ABCB8 is essential for normal cardiac function, maintenance of mitochondrial iron homeostasis and maturation of cytosolic Fe/S proteins. In summary, this report provides characterization of a protein involved in mitochondrial iron export.

Original languageEnglish (US)
Pages (from-to)4152-4157
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number11
DOIs
StatePublished - Mar 13 2012

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Cardiomyopathies
Iron
Adenosine Triphosphate
Protein S
Mitochondria
ATP-Binding Cassette Transporters
Heme
Sulfur
Echocardiography
Reactive Oxygen Species
Membrane Proteins
Oxidative Stress
Homeostasis
Cell Death
Down-Regulation
Hemodynamics
Maintenance
Enzymes
Proteins

ASJC Scopus subject areas

  • General

Cite this

Ichikawa, Yoshihiko ; Bayeva, Marina ; Ghanefar, Mohsen ; Potini, Vishnu ; Sun, Lin ; Mutharasan, R Kannan ; Wu, Rongxue ; Khechaduri, Arineh ; Naik, Tejaswitha Jairaj ; Ardehali, Hossein. / Disruption of ATP-binding cassette B8 in mice leads to cardiomyopathy through a decrease in mitochondrial iron export. In: Proceedings of the National Academy of Sciences of the United States of America. 2012 ; Vol. 109, No. 11. pp. 4152-4157.
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abstract = "Mitochondrial iron levels are tightly regulated, as iron is essential for the synthesis of Fe/S clusters and heme in the mitochondria, but high levels can cause oxidative stress. The ATP-binding cassette (ABC) transporter ABCB8 is a mitochondrial inner membrane protein with an unknown function. Here, we show that ABCB8 is involved in mitochondrial iron export and is essential for baseline cardiac function. Induced genetic deletion of ABCB8 in mouse hearts resulted in mitochondrial iron accumulation and cardiomyopathy, as assessed by echocardiography and invasive hemodynamics. Mice with ABCB8 deletion in the heart also displayed mitochondrial damage, and higher levels of reactive oxygen species and cell death. Down-regulation of ABCB8 in vitro resulted in decreased iron export from isolated mitochondria, whereas its overexpression had the opposite effect. Furthermore, ABCB8 is needed for the maturation of the cytosolic Fe/S proteins, as its deletion in vitro and in vivo led to decreased activity of cytosolic, but not mitochondrial, iron-sulfur-containing enzymes. These results indicate that ABCB8 is essential for normal cardiac function, maintenance of mitochondrial iron homeostasis and maturation of cytosolic Fe/S proteins. In summary, this report provides characterization of a protein involved in mitochondrial iron export.",
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Disruption of ATP-binding cassette B8 in mice leads to cardiomyopathy through a decrease in mitochondrial iron export. / Ichikawa, Yoshihiko; Bayeva, Marina; Ghanefar, Mohsen; Potini, Vishnu; Sun, Lin; Mutharasan, R Kannan; Wu, Rongxue; Khechaduri, Arineh; Naik, Tejaswitha Jairaj; Ardehali, Hossein.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 11, 13.03.2012, p. 4152-4157.

Research output: Contribution to journalArticle

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AU - Ichikawa, Yoshihiko

AU - Bayeva, Marina

AU - Ghanefar, Mohsen

AU - Potini, Vishnu

AU - Sun, Lin

AU - Mutharasan, R Kannan

AU - Wu, Rongxue

AU - Khechaduri, Arineh

AU - Naik, Tejaswitha Jairaj

AU - Ardehali, Hossein

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