Disruption of Bardet-Biedl syndrome ciliary proteins perturbs planar cell polarity in vertebrates

Alison J. Ross, Helen May-Simera, Erica R. Eichers, Masatake Kai, Josephine Hill, Daniel J. Jagger, Carmen C. Leitch, J. Paul Chapple, Peter M. Munro, Shannon Fisher, Perciliz L. Tan, Helen M. Phillips, Michel R. Leroux, Deborah J. Henderson, Jennifer N. Murdoch, Andrew J. Copp, Marie Madeleine Eliot, James R. Lupski, David T. Kemp, Hélène DollfusMasazumi Tada, Elias Nicholas Katsanis, Andrew Forge, Philip L. Beales

Research output: Contribution to journalArticlepeer-review

460 Scopus citations

Abstract

The evolutionarily conserved planar cell polarity (PCP) pathway (or noncanonical Wnt pathway) drives several important cellular processes, including epithelial cell polarization, cell migration and mitotic spindle orientation. In vertebrates, PCP genes have a vital role in polarized convergent extension movements during gastrulation and neurulation. Here we show that mice with mutations in genes involved in Bardet-Biedl syndrome (BBS), a disorder associated with ciliary dysfunction, share phenotypes with PCP mutants including open eyelids, neural tube defects and disrupted cochlear stereociliary bundles. Furthermore, we identify genetic interactions between BBS genes and a PCP gene in both mouse (Ltap, also called Vangl2) and zebrafish (vangl2). In zebrafish, the augmented phenotype results from enhanced defective convergent extension movements. We also show that Vangl2 localizes to the basal body and axoneme of ciliated cells, a pattern reminiscent of that of the BBS proteins. These data suggest that cilia are intrinsically involved in PCP processes.

Original languageEnglish (US)
Pages (from-to)1135-1140
Number of pages6
JournalNature Genetics
Volume37
Issue number10
DOIs
StatePublished - Oct 2005

ASJC Scopus subject areas

  • Genetics

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