Dissecting receptor-G protein specificity using Gα chimeras

Theresa M. Cabrera-Vera*, Tarita O. Thomas, Jurgen Vanhauwe, Karyn M. Depree, Stephen G. Graber, Heidi E. Hamm

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

In conclusion, by taking advantage of the overall sequence homology and structural similarity of Gα subunits, functional chimeric Gα subunits can be generated and used as tools for the identification of sequence-specific factors that mediate receptor: G protein specificity. The [35S] GTPγS binding assay and the affinity shift activity assay are two sensitive biochemical approaches that can be used to assess receptor: G protein coupling in vitro. These in vitro assays limit confounding influences from cellular proteins and allow for the strict control of receptor: G protein ratios.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc
Pages69-81
Number of pages13
DOIs
StatePublished - 2002

Publication series

NameMethods in Enzymology
Volume344
ISSN (Print)0076-6879

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

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