@inbook{dad8c73ec5d041afac564461ea9c7f44,
title = "Dissecting receptor-G protein specificity using Gα chimeras",
abstract = "In conclusion, by taking advantage of the overall sequence homology and structural similarity of Gα subunits, functional chimeric Gα subunits can be generated and used as tools for the identification of sequence-specific factors that mediate receptor: G protein specificity. The [35S] GTPγS binding assay and the affinity shift activity assay are two sensitive biochemical approaches that can be used to assess receptor: G protein coupling in vitro. These in vitro assays limit confounding influences from cellular proteins and allow for the strict control of receptor: G protein ratios.",
author = "Cabrera-Vera, {Theresa M.} and Thomas, {Tarita O.} and Jurgen Vanhauwe and Depree, {Karyn M.} and Graber, {Stephen G.} and Hamm, {Heidi E.}",
year = "2002",
doi = "10.1016/S0076-6879(02)44706-4",
language = "English (US)",
series = "Methods in Enzymology",
publisher = "Academic Press Inc",
pages = "69--81",
booktitle = "Methods in Enzymology",
}