Dissociation of specific binding of 25-OH-D3 and resorption in fetal rat bones

A. M. Mellow; G. V. Stosich; P. H. Stern

doi:10.1016/0303-7207(78)90121-1

Dissociation of specific binding of 25-OH-D₃ and resorption in fetal rat bones

A. M. Mellow^*, G. V. Stosich, P. H. Stern

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Specific binding of 25-OH-D₃ was measured in cytosol prepared from isolated fetal rat bone cells. Binding was significant after 2 h incubation at 0°C and appeared to be approaching a plateau at 4 h. Binding was half-maximal at 1.7 × 10^-10 M 25-OH-D₃. 24(R),25-(OH)₂D₃, which was approximately equipotent with 25-OH-D₃ in bone culture, had approximately the same binding activity. 1,25-(OH)₂D₃, which was 2-3 orders of magnitude more active on resorption, was a much weaker competitor for binding. Vitamin D₃, which was inactive in culture, was at least as effective a competitor as 1,25-(OH)₂D₃. The results suggest that the cytosol site which specifically binds 25-OH-D₃ is not the mediator of the bone-resorbing activity.

Original language	English (US)
Pages (from-to)	149-158
Number of pages	10
Journal	Molecular and Cellular Endocrinology
Volume	10
Issue number	2
DOIs	https://doi.org/10.1016/0303-7207(78)90121-1
State	Published - Apr 1978

Funding

* Supported by NIH Research Grant AM11262 and NIH Research Career Development Award 5 K04 AM70210 (to P.H.S.). A preliminary report of these findings was presented at the 1976 Fall Meetings of the American Society for Pharmacology and Experimental Therapeutics (Pharmacologist 18, 233, 1976). The bone resorption data only were included in studies presented at the Third Workshop on Vitamin D at Asilomar, Calif., U.S.A., Jan. 1977 (Vitamin D: Biochemical, Chemical and Clinical Aspects Related to Calcium Metabolism, Walter de Gruyter, Berlin-New York, 1977, pp. 531-540).

Keywords

25-hydroxy vitamin D
25-hydroxyvitamin D receptor
bone cells
bone cytosol
bone resorption
vitamin D

ASJC Scopus subject areas

Endocrinology
Molecular Biology
Biochemistry

Access to Document

10.1016/0303-7207(78)90121-1

Cite this

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title = "Dissociation of specific binding of 25-OH-D3 and resorption in fetal rat bones",

abstract = "Specific binding of 25-OH-D3 was measured in cytosol prepared from isolated fetal rat bone cells. Binding was significant after 2 h incubation at 0°C and appeared to be approaching a plateau at 4 h. Binding was half-maximal at 1.7 × 10-10 M 25-OH-D3. 24(R),25-(OH)2D3, which was approximately equipotent with 25-OH-D3 in bone culture, had approximately the same binding activity. 1,25-(OH)2D3, which was 2-3 orders of magnitude more active on resorption, was a much weaker competitor for binding. Vitamin D3, which was inactive in culture, was at least as effective a competitor as 1,25-(OH)2D3. The results suggest that the cytosol site which specifically binds 25-OH-D3 is not the mediator of the bone-resorbing activity.",

keywords = "25-hydroxy vitamin D, 25-hydroxyvitamin D receptor, bone cells, bone cytosol, bone resorption, vitamin D",

author = "Mellow, {A. M.} and Stosich, {G. V.} and Stern, {P. H.}",

note = "Funding Information: * Supported by NIH Research Grant AM11262 and NIH Research Career Development Award 5 K04 AM70210 (to P.H.S.). A preliminary report of these findings was presented at the 1976 Fall Meetings of the American Society for Pharmacology and Experimental Therapeutics (Pharmacologist 18, 233, 1976). The bone resorption data only were included in studies presented at the Third Workshop on Vitamin D at Asilomar, Calif., U.S.A., Jan. 1977 (Vitamin D: Biochemical, Chemical and Clinical Aspects Related to Calcium Metabolism, Walter de Gruyter, Berlin-New York, 1977, pp. 531-540).",

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N2 - Specific binding of 25-OH-D3 was measured in cytosol prepared from isolated fetal rat bone cells. Binding was significant after 2 h incubation at 0°C and appeared to be approaching a plateau at 4 h. Binding was half-maximal at 1.7 × 10-10 M 25-OH-D3. 24(R),25-(OH)2D3, which was approximately equipotent with 25-OH-D3 in bone culture, had approximately the same binding activity. 1,25-(OH)2D3, which was 2-3 orders of magnitude more active on resorption, was a much weaker competitor for binding. Vitamin D3, which was inactive in culture, was at least as effective a competitor as 1,25-(OH)2D3. The results suggest that the cytosol site which specifically binds 25-OH-D3 is not the mediator of the bone-resorbing activity.

AB - Specific binding of 25-OH-D3 was measured in cytosol prepared from isolated fetal rat bone cells. Binding was significant after 2 h incubation at 0°C and appeared to be approaching a plateau at 4 h. Binding was half-maximal at 1.7 × 10-10 M 25-OH-D3. 24(R),25-(OH)2D3, which was approximately equipotent with 25-OH-D3 in bone culture, had approximately the same binding activity. 1,25-(OH)2D3, which was 2-3 orders of magnitude more active on resorption, was a much weaker competitor for binding. Vitamin D3, which was inactive in culture, was at least as effective a competitor as 1,25-(OH)2D3. The results suggest that the cytosol site which specifically binds 25-OH-D3 is not the mediator of the bone-resorbing activity.

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