Distance-dependent photoinduced electron transfer in synthetic single- strand and hairpin DNA

Frederick D. Lewis*, Robert L. Letsinger

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

The singlet state of stilbene-4,4'-dicarboxamide can serve as a fluorescent probe of both DNA conformation and electron transfer. Covalent incorporation of the stilbene-dicarboxamide into DNA structures with restricted conformational mobility results in inhibition of stilbene isomerization and an increase in its fluorescence quantum yield and lifetime. The fluorescence of stilbenedicarboxamide is selectively quenched by proximate guanine, but not by the three other DNA nucleobases. Selective quenching occurs via an electron transfer mechanism in which stilbene serves as the electron acceptor and guanine as the electron donor. Kinetic analysis of the distance dependence of electron transfer in stilbene-bridged hairpins suggests that duplex DNA is more effective than proteins as a medium for electron transfer, but that it does not function as a molecular wire.

Original languageEnglish (US)
Pages (from-to)215-221
Number of pages7
JournalJournal of Biological Inorganic Chemistry
Volume3
Issue number2
DOIs
StatePublished - Apr 1 1998

Keywords

  • DNA
  • Electron transfer
  • Guanine
  • Radical ions
  • Stilbene

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

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