Distinct developmental subtypes of cultured non-stellate rat astrocytes distinguished by a new glial intermediate filament-associated protein

Hsi Yuang Yang*, Norman Lieska, Robert D. Goldman, Diane Johnson-Seaton, George D. Pappas

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The nature and tissue origin of cultured non-stellate astrocytes have not been defined. On the basis of immunofluorescence microscopy using multiple double-labeling with antibodies to glial fibrillary acidic protein (GFAP), vimentin, and the recently identified intermediate filament-associated protein (IFAP)-70/280kD, four distinct astrocytic subtypes were definable in neonatal rat brain astrocytes in culture. All of these were of the non-stellate type on the basis of morphology. Similar examination of developing rat cerebral cortex identified these same subtypes as distinct differentiation states of astrocytes. These findings indicate that the parallel developmental events can be studied in vitro.

Original languageEnglish (US)
Pages (from-to)161-168
Number of pages8
JournalBrain research
Volume573
Issue number1
DOIs
StatePublished - Feb 21 1992

Keywords

  • Glial fibrillary acidic protein
  • Intermediate filament
  • Intermediate filament-associated protein
  • Non-stellate astrocyte
  • Radial glia
  • Vimentin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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