Distinctive nitrogen oxides reduction capabilities of a Denitrifying Polyphosphate Accumulating Organisms (DPAOs) enrichment in a long-term nitrite fed sequencing batch reactor

Han Gao, Xiaotian Zhao, Lei Zhou, George F. Wells

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Denitrifying polyphosphate accumulating organisms (DPAOs), are capable of nitrate and/or nitrite (NO2-) reduction coupled to P uptake when subjected to alternating anaerobic/anoxic conditions. However, strong accumulation of nitrous oxide (N2O) has been previously observed in DPAO enrichments under high NO2- concentrations. To date, denitrification capability and denitrifying P uptake rates of DPAOs using different electron acceptors after long-term exposure to elevated NO2- concentration hasn't been examined. To address this knowledge gap, we operated a lab-scale SBR under alternating anaerobic/anoxic conditions with high nitrite feed for >2 years. Ex-situ batch assays were performed to clarify capacity for reduction of various nitrogen oxides and P uptake of the DPAOs enrichment culture. These batch assays revealed distinct nitrogen oxides reduction and denitrifying P uptake capabilities. High-throughput 16S rRNA sequencing combined with qPCR assays revealed the selection of putative DPAOs and other potential denitrifying microbial consortia responsible for N2O accumulation under high nitrite-feeding regimes.

Original languageEnglish (US)
Title of host publicationWater Environment Federation Technical Exhibition and Conference 2017, WEFTEC 2017
PublisherWater Environment Federation
Pages1755-1778
Number of pages24
ISBN (Print)9781510863682
StatePublished - Jan 1 2017
EventWater Environment Federation Technical Exhibition and Conference 2017, WEFTEC 2017 - Chicago, United States
Duration: Sep 30 2017Oct 4 2017

Publication series

NameWater Environment Federation Technical Exhibition and Conference 2017, WEFTEC 2017
Volume3

Other

OtherWater Environment Federation Technical Exhibition and Conference 2017, WEFTEC 2017
CountryUnited States
CityChicago
Period9/30/1710/4/17

Keywords

  • DPAOs
  • Denitrification
  • Granular biomass
  • Nitrite

ASJC Scopus subject areas

  • Pollution
  • Ecological Modeling
  • Environmental Chemistry
  • Waste Management and Disposal
  • Water Science and Technology

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