Distribution and characterization of hexose 6-phosphate dehydrogenase in trout

John J. Stegeman; Erwin Goldberg

doi:10.1007/BF00485675

Distribution and characterization of hexose 6-phosphate dehydrogenase in trout

John J. Stegeman^*, Erwin Goldberg

^*Corresponding author for this work

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Electrophoretic analysis of trout liver extracts indicates that an autosomal gene coding for hexose 6-phosphate dehydrogenase (H6P D) in lake trout, Hpd-L, is monomorphic. In brook trout, the gene is polymorphic, having at least three genetic variants termed Hpd-B¹, Hpd-B², and Hpd-B³. F₁ hybrid splake exhibit the three expected phenotypes resulting from Hpd-L/Hpd-B¹, Hpd-L/Hpd-B², and Hpd-L/Hpd-B³ genotypes. Trout H6P D is ostensibly a dimer of mol wt 230,000. The characteristics of trout H6P D, including substrate specificity, genetic polymorphism, electrophoretic characteristics, subcellular localization, and tissue distribution are in close agreement with results obtained for mammalian H6P D. We suggest that trout and mammalian H6PDs, X-linked mammalian G6P D, and autosomal avian G6P D arose from a common ancestral type G6P D.

Original language	English (US)
Pages (from-to)	579-589
Number of pages	11
Journal	Biochemical Genetics
Volume	5
Issue number	6
DOIs	https://doi.org/10.1007/BF00485675
State	Published - Dec 1 1971

ASJC Scopus subject areas

Biochemistry
Ecology, Evolution, Behavior and Systematics
Molecular Biology
Genetics

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title = "Distribution and characterization of hexose 6-phosphate dehydrogenase in trout",

abstract = "Electrophoretic analysis of trout liver extracts indicates that an autosomal gene coding for hexose 6-phosphate dehydrogenase (H6P D) in lake trout, Hpd-L, is monomorphic. In brook trout, the gene is polymorphic, having at least three genetic variants termed Hpd-B1, Hpd-B2, and Hpd-B3. F1 hybrid splake exhibit the three expected phenotypes resulting from Hpd-L/Hpd-B1, Hpd-L/Hpd-B2, and Hpd-L/Hpd-B3 genotypes. Trout H6P D is ostensibly a dimer of mol wt 230,000. The characteristics of trout H6P D, including substrate specificity, genetic polymorphism, electrophoretic characteristics, subcellular localization, and tissue distribution are in close agreement with results obtained for mammalian H6P D. We suggest that trout and mammalian H6PDs, X-linked mammalian G6P D, and autosomal avian G6P D arose from a common ancestral type G6P D.",

author = "Stegeman, {John J.} and Erwin Goldberg",

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doi = "10.1007/BF00485675",

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TY - JOUR

T1 - Distribution and characterization of hexose 6-phosphate dehydrogenase in trout

AU - Stegeman, John J.

AU - Goldberg, Erwin

PY - 1971/12/1

Y1 - 1971/12/1

N2 - Electrophoretic analysis of trout liver extracts indicates that an autosomal gene coding for hexose 6-phosphate dehydrogenase (H6P D) in lake trout, Hpd-L, is monomorphic. In brook trout, the gene is polymorphic, having at least three genetic variants termed Hpd-B1, Hpd-B2, and Hpd-B3. F1 hybrid splake exhibit the three expected phenotypes resulting from Hpd-L/Hpd-B1, Hpd-L/Hpd-B2, and Hpd-L/Hpd-B3 genotypes. Trout H6P D is ostensibly a dimer of mol wt 230,000. The characteristics of trout H6P D, including substrate specificity, genetic polymorphism, electrophoretic characteristics, subcellular localization, and tissue distribution are in close agreement with results obtained for mammalian H6P D. We suggest that trout and mammalian H6PDs, X-linked mammalian G6P D, and autosomal avian G6P D arose from a common ancestral type G6P D.

AB - Electrophoretic analysis of trout liver extracts indicates that an autosomal gene coding for hexose 6-phosphate dehydrogenase (H6P D) in lake trout, Hpd-L, is monomorphic. In brook trout, the gene is polymorphic, having at least three genetic variants termed Hpd-B1, Hpd-B2, and Hpd-B3. F1 hybrid splake exhibit the three expected phenotypes resulting from Hpd-L/Hpd-B1, Hpd-L/Hpd-B2, and Hpd-L/Hpd-B3 genotypes. Trout H6P D is ostensibly a dimer of mol wt 230,000. The characteristics of trout H6P D, including substrate specificity, genetic polymorphism, electrophoretic characteristics, subcellular localization, and tissue distribution are in close agreement with results obtained for mammalian H6P D. We suggest that trout and mammalian H6PDs, X-linked mammalian G6P D, and autosomal avian G6P D arose from a common ancestral type G6P D.

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Distribution and characterization of hexose 6-phosphate dehydrogenase in trout

Abstract

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