Diverse TCRs recognize murine CD1

Samuel M. Behar*, T. A. Podrebarac, C. J. Roy, C. R. Wang, M. B. Brenner

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

174 Scopus citations


Human and murine T cells that specifically recognize CD1d and produce IL-4 and IFN-γ, play a role in immunoregulation and tumor rejection. In the mouse, most CD1d1-reactive T cells described express an invariant Vα14- Jα281 TCR associated with TCR β-chains of limited diversity. Similarly, human CD1d-reactive T cells express a highly restricted TCR repertoire. Here we report the unexpected result that in mice immunized with CD1d1-bearing transfectant cells, a diverse repertoire of TCRs was expressed by CD1d1- reactive T cell clones isolated by limiting dilution without preselection for NK1 expression. Only 3 of 10 CD1d1-reactive T cell clones expressed the invariant Vα14-Jα281 TCRα rearrangement. T cells expressing Vα10, -11, - 15, and -17, and having non-germline-encoded nucleotides resulting in diverse V-J junctions were identified. Like CD1d1-reactive T cells expressing the invariant Vα14-Jα281 TCR α-chain, CD1d1-reactive clones with diverse TCRs produced both Type 1 (IFN-γ) and Type 2 (IL-4, IL-10) cytokines. This establishes the existence of significant diversity in the TCRs directly reactive to the CD1d1 protein. Our findings reveal that CD1d interacts with a broad array of TCRs, suggesting substantial redundancy and flexibility of the immune system in providing T cells serving the role(s) mediated by CD1d reactivity.

Original languageEnglish (US)
Pages (from-to)161-167
Number of pages7
JournalJournal of Immunology
Issue number1
StatePublished - Jan 1 1999

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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