DNA 5-Methylcytosine-Specific Amplification and Sequencing

Chang Liu; Xiaolong Cui; Boxuan Simen Zhao; Pradnya Narkhede; Yawei Gao; Jun Liu; Xiaoyang Dou; Qing Dai; Li Sheng Zhang; Chuan He

doi:10.1021/jacs.9b12707

DNA 5-Methylcytosine-Specific Amplification and Sequencing

Chang Liu, Xiaolong Cui, Boxuan Simen Zhao, Pradnya Narkhede, Yawei Gao, Jun Liu, Xiaoyang Dou, Qing Dai, Li Sheng Zhang, Chuan He^*

^*Corresponding author for this work

Preventive Medicine

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

DNA 5-methylcytosine (5mC)-specific mapping has been hampered by severe DNA degradation and the presence of 5-hydroxymethylcytosine (5hmC) using the conventional bisulfite sequencing approach. Here, we present a 5mC-specific whole-genome amplification method (5mC-WGA), with which we achieved 5mC retention during DNA amplification from limited input down to 10 pg scale with limited interference from 5hmC signals, providing DNA 5mC methylome with high reproducibility and accuracy.

Original language	English (US)
Pages (from-to)	4539-4543
Number of pages	5
Journal	Journal of the American Chemical Society
Volume	142
Issue number	10
DOIs	https://doi.org/10.1021/jacs.9b12707
State	Published - Mar 11 2020

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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title = "DNA 5-Methylcytosine-Specific Amplification and Sequencing",

abstract = "DNA 5-methylcytosine (5mC)-specific mapping has been hampered by severe DNA degradation and the presence of 5-hydroxymethylcytosine (5hmC) using the conventional bisulfite sequencing approach. Here, we present a 5mC-specific whole-genome amplification method (5mC-WGA), with which we achieved 5mC retention during DNA amplification from limited input down to 10 pg scale with limited interference from 5hmC signals, providing DNA 5mC methylome with high reproducibility and accuracy.",

author = "Chang Liu and Xiaolong Cui and Zhao, {Boxuan Simen} and Pradnya Narkhede and Yawei Gao and Jun Liu and Xiaoyang Dou and Qing Dai and Zhang, {Li Sheng} and Chuan He",

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year = "2020",

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doi = "10.1021/jacs.9b12707",

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T1 - DNA 5-Methylcytosine-Specific Amplification and Sequencing

AU - Liu, Chang

AU - Cui, Xiaolong

AU - Zhao, Boxuan Simen

AU - Narkhede, Pradnya

AU - Gao, Yawei

AU - Liu, Jun

AU - Dou, Xiaoyang

AU - Dai, Qing

AU - Zhang, Li Sheng

AU - He, Chuan

PY - 2020/3/11

Y1 - 2020/3/11

N2 - DNA 5-methylcytosine (5mC)-specific mapping has been hampered by severe DNA degradation and the presence of 5-hydroxymethylcytosine (5hmC) using the conventional bisulfite sequencing approach. Here, we present a 5mC-specific whole-genome amplification method (5mC-WGA), with which we achieved 5mC retention during DNA amplification from limited input down to 10 pg scale with limited interference from 5hmC signals, providing DNA 5mC methylome with high reproducibility and accuracy.

AB - DNA 5-methylcytosine (5mC)-specific mapping has been hampered by severe DNA degradation and the presence of 5-hydroxymethylcytosine (5hmC) using the conventional bisulfite sequencing approach. Here, we present a 5mC-specific whole-genome amplification method (5mC-WGA), with which we achieved 5mC retention during DNA amplification from limited input down to 10 pg scale with limited interference from 5hmC signals, providing DNA 5mC methylome with high reproducibility and accuracy.

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JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

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DNA 5-Methylcytosine-Specific Amplification and Sequencing

Abstract

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