DNA at aqueous/solid interfaces: Chirality-based detection via second harmonic generation activity

Faith C. Boman, Julianne M. Gibbs-Davis, Laurel M. Heckman, Brian R. Stepp, Sonbinh T. Nguyen, Franz M. Geiger

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

We present electronic spectra of single-strand and duplex DNA oligonucleotides covalently attached to fused quartz/aqueous interfaces and demonstrate that a strong nonlinear optical linear dichroism response is obtained when adenine and thymine bases undergo Watson-Crick base pairing to form a double helix. Complementary χ (3) charge screening studies indicate that the signal originates from 5 × 10 11 strands per square centimeter, or 6 attomoles of surface-bound oligonucleotides. The label-free, molecular-specific nature afforded by nonlinear optical studies of DNA at aqueous/solid interfaces allows for the real-time tracking of interfacial DNA hybridization for the first time.

Original languageEnglish (US)
Pages (from-to)844-848
Number of pages5
JournalJournal of the American Chemical Society
Volume131
Issue number2
DOIs
StatePublished - Jan 21 2009

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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