DNA-protein interactions in the CCAAT box region of the murine lactate dehydrogenase C promoter

Madhavee Ambhaikar, Erwin Goldberg*

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Electrophoretic Mobility (EMSA), using oligonucleotides containing CCAAT box sequences from the murine Idhc promoter show the presence of CCAAT binding proteins in nuclear extracts from liver and testis. In a liver extract, a single shifted band is seen. However, in the testis extract, two shifts are observed, one of which may be due to a testis specific isoform of CCAAT binding factor (CBF). Southwestern analysis with an oligonucleotide probe containing these sequences reveals the presence of a protein of approximately 120 kD in the testis extract. In the liver extract, a 70-kD protein binds the probe. An antibody against HeLa CBF causes a supershift in testis nuclear extract.

Original languageEnglish (US)
Pages (from-to)360-365
Number of pages6
JournalMolecular reproduction and development
Volume52
Issue number4
DOIs
StatePublished - 1999

Keywords

  • CCAAT box binding factor
  • Gel shift
  • Testis

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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