DNA Sequence, genomic organization, and chromosomal localization of the mouse peripheral myelin protein zero gene: Identification of polymorphic alleles

Kwan Hee You; Chih Lin Hsieh; Carol Hayes; Norbert Stahl; Uta Francke; Brian Popko

doi:10.1016/0888-7543(91)90370-T

DNA Sequence, genomic organization, and chromosomal localization of the mouse peripheral myelin protein zero gene: Identification of polymorphic alleles

Kwan Hee You, Chih Lin Hsieh, Carol Hayes, Norbert Stahl, Uta Francke, Brian Popko^*

^*Corresponding author for this work

Neurology

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

We have cloned and characterized the mouse gene, P₀, that encodes the predominant protein of peripheral myelin. Similar to the rat gene, the mouse P₀ gene is encoded by six exons that span about 7 kb of DNA. The DNA sequence of the mouse gene is highly homologous with the rat gene, including the regions believed to be important in transcriptional control. Furthermore, the P₀ protein appears well conserved throughout evolution. The gene was mapped to mouse chromosome 1 by Southern analysis of a Chinese hamster × mouse somatic cell hybrid panel. Several polymorphic restriction enzyme sites were identified within the P₀ locus. Recombinant inbred strain mapping has linked the P₀ gene to Ly-9/Ly-17/Sap in a region corresponding to band 1H3.

Original language	English (US)
Pages (from-to)	751-757
Number of pages	7
Journal	Genomics
Volume	9
Issue number	4
DOIs	https://doi.org/10.1016/0888-7543(91)90370-T
State	Published - Apr 1991

ASJC Scopus subject areas

Genetics

Access to Document

10.1016/0888-7543(91)90370-T

Cite this

@article{89ac40a198d0498b95676955653f6c01,

title = "DNA Sequence, genomic organization, and chromosomal localization of the mouse peripheral myelin protein zero gene: Identification of polymorphic alleles",

abstract = "We have cloned and characterized the mouse gene, P0, that encodes the predominant protein of peripheral myelin. Similar to the rat gene, the mouse P0 gene is encoded by six exons that span about 7 kb of DNA. The DNA sequence of the mouse gene is highly homologous with the rat gene, including the regions believed to be important in transcriptional control. Furthermore, the P0 protein appears well conserved throughout evolution. The gene was mapped to mouse chromosome 1 by Southern analysis of a Chinese hamster × mouse somatic cell hybrid panel. Several polymorphic restriction enzyme sites were identified within the P0 locus. Recombinant inbred strain mapping has linked the P0 gene to Ly-9/Ly-17/Sap in a region corresponding to band 1H3.",

author = "You, {Kwan Hee} and Hsieh, {Chih Lin} and Carol Hayes and Norbert Stahl and Uta Francke and Brian Popko",

note = "Funding Information: We thank Dr. (+reg Lemke for his kind gift of the rat P, cDNA. We also appreciate the fine technical assistance of Stephanie Shouse. This work was supported in part by the National Multiple Sclerosis Society Research Grant RG 2089Al; the 1J.S. Public Health Service Research Grants R29 NS273.16, ESO1104. and GM26105; and the LJ.S. Public Health Service Mental Retardation Research Center Core Grant P30 HD-031 IO. U.F. is an investigator and C.-L.H. is an associate of the Howard Hughes Medical Institute.",

year = "1991",

month = apr,

doi = "10.1016/0888-7543(91)90370-T",

language = "English (US)",

volume = "9",

pages = "751--757",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press Inc.",

number = "4",

}

TY - JOUR

T1 - DNA Sequence, genomic organization, and chromosomal localization of the mouse peripheral myelin protein zero gene

T2 - Identification of polymorphic alleles

AU - You, Kwan Hee

AU - Hsieh, Chih Lin

AU - Hayes, Carol

AU - Stahl, Norbert

AU - Francke, Uta

AU - Popko, Brian

N1 - Funding Information: We thank Dr. (+reg Lemke for his kind gift of the rat P, cDNA. We also appreciate the fine technical assistance of Stephanie Shouse. This work was supported in part by the National Multiple Sclerosis Society Research Grant RG 2089Al; the 1J.S. Public Health Service Research Grants R29 NS273.16, ESO1104. and GM26105; and the LJ.S. Public Health Service Mental Retardation Research Center Core Grant P30 HD-031 IO. U.F. is an investigator and C.-L.H. is an associate of the Howard Hughes Medical Institute.

PY - 1991/4

Y1 - 1991/4

N2 - We have cloned and characterized the mouse gene, P0, that encodes the predominant protein of peripheral myelin. Similar to the rat gene, the mouse P0 gene is encoded by six exons that span about 7 kb of DNA. The DNA sequence of the mouse gene is highly homologous with the rat gene, including the regions believed to be important in transcriptional control. Furthermore, the P0 protein appears well conserved throughout evolution. The gene was mapped to mouse chromosome 1 by Southern analysis of a Chinese hamster × mouse somatic cell hybrid panel. Several polymorphic restriction enzyme sites were identified within the P0 locus. Recombinant inbred strain mapping has linked the P0 gene to Ly-9/Ly-17/Sap in a region corresponding to band 1H3.

AB - We have cloned and characterized the mouse gene, P0, that encodes the predominant protein of peripheral myelin. Similar to the rat gene, the mouse P0 gene is encoded by six exons that span about 7 kb of DNA. The DNA sequence of the mouse gene is highly homologous with the rat gene, including the regions believed to be important in transcriptional control. Furthermore, the P0 protein appears well conserved throughout evolution. The gene was mapped to mouse chromosome 1 by Southern analysis of a Chinese hamster × mouse somatic cell hybrid panel. Several polymorphic restriction enzyme sites were identified within the P0 locus. Recombinant inbred strain mapping has linked the P0 gene to Ly-9/Ly-17/Sap in a region corresponding to band 1H3.

UR - http://www.scopus.com/inward/record.url?scp=0025971851&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025971851&partnerID=8YFLogxK

U2 - 10.1016/0888-7543(91)90370-T

DO - 10.1016/0888-7543(91)90370-T

M3 - Article

C2 - 1709914

AN - SCOPUS:0025971851

SN - 0888-7543

VL - 9

SP - 751

EP - 757

JO - Genomics

JF - Genomics

IS - 4

ER -

DNA Sequence, genomic organization, and chromosomal localization of the mouse peripheral myelin protein zero gene: Identification of polymorphic alleles

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this