DNA sequence recognition by hybridization to short oligomers: Experimental verification of the method on the E. coli genome

Aleksandar Milosavljević*, Suzana Savković, Radomir Crkvenjakov, David Salbego, Hope Serrato, Heidi Kreuzer, Anne Gemmell, Shawna Batus, Danica Grujić, Susan Carnahan, Tatjana Paunesku, Jovanka Tepavčević

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

A newly developed method for sequence recognition by hybridization to short oligomers is verified for the first time in genome-scale experiments. The experiments involved hybridization of 15,328 randomly selected 2-kb genomic clones of Escherichia coli with 997 short oligomer probes to detect complementary oligomers within the clones. Lists of oligomers detected within individual clones were compiled into a database. The database was then searched using known E. coli sequences as queries. The goal was to recognize the clones that are identical or similar to the query sequences. A total of 76 putative recognitions were tested in two separate but complementary recognition experiments. The results indicate high specificity of recognition. Current and prospective applications of this novel method are discussed.

Original languageEnglish (US)
Pages (from-to)77-86
Number of pages10
JournalGenomics
Volume37
Issue number1
DOIs
StatePublished - Oct 1 1996

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'DNA sequence recognition by hybridization to short oligomers: Experimental verification of the method on the E. coli genome'. Together they form a unique fingerprint.

Cite this