Domain-specific monoclonal antibodies produced against human PGRN

Lihong Chai, Qinwen Mao, Shihai Liu, Haibin Xia*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Progranulin (PGRN) encodes a 68.5-kDa secreted growth factor that is composed of seven and a half tandem repeats of a 12-cysteine granulin motif. PGRN is expressed in many tissues and has a role in mediating development, wound repair, inflammation, and tumorigenesis. Mutations leading to a loss of function in PGRN are the most common cause of familial frontotemporal lobar degeneration with TDP-43-positive inclusions (FTLD-TDP). In this study, we established hybridoma cell lines producing antibodies against human PGRN. Murine monoclonal antibodies (MAbs) against human PGRN were generated by using purified eukaryotic recombinant PGRN-6His fusion protein as immunogen. A panel of seven monoclonal antibodies was obtained after the preliminary screening by indirect enzyme-linked immunosorbent assay (ELISA), the data of which was confirmed by Western blot analysis and immunocytochemistry. By using constructs expressing a series of C- and N-terminal truncations, and single domains of PGRN, the particular domains recognized by MAbs were also identified. Domain-specific anti-PGRN MAbs will be an essential tool for investigating the role of PGRN in normal physiological or pathological conditions.

Original languageEnglish (US)
Pages (from-to)271-278
Number of pages8
Issue number3
StatePublished - Jun 1 2011

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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