Dopamine restores lung ability to clear edema in rats exposed to hyperoxia

Fernando J. Saldías, Emilia Lecuona, Alejandro P. Comellas, Karen M. Ridge, Jacob I. Sznajder*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

56 Scopus citations


Exposure to hyperoxia causes lung injury, decreases active sodium transport and lung edema clearance in rats. Dopamine (DA) increases lung edema clearance by stimulating vectorial Na+ flux and Na,K-ATPase function in rat alveolar epithelium. This study was designed to test whether DA (10- 5 M) would increase lung edema clearance in rats exposed to 100% O2 for 64 h. Active Na+ transport and lung edema clearance decreased by approximately 44% in rats exposed to acute hyperoxia (p < 0.001). DA increased lung edema clearance in room air breathing rats (from 0.50 ± 0.02 to 0.75 ± 0.06 ml/h) and in rats exposed to 100% O2 (from 0.28 ± 0.03 to 0.67 ± 0.03 ml/h). Disruption of cell microtubular transport system by colchicine blocked the stimulatory effect of DA on active Na+ transport in control and hyperoxic rats, whereas the isomer β-lumicolchicine, which does not affect cell microtubular transport, did not inhibit the stimulatory effects of dopamine. The Na,K-ATPase α1-subunit protein abundance increased in the basolateral membranes of alveolar type II (ATII) cells incubated with 10-5 M DA for 15 min, probably by recruiting Na+ pumps from intracellular pools. Colchicine, but not β-lumicolchicine, prevented the recruitment of α1 subunits to the plasma membrane by DA. Accordingly, DA restored lung ability to clear edema in hyperoxic-injured rat lungs. Conceivably, dopamine induces recruitment of Na+ pumps from intracellular pools to the plasma membrane of alveolar epithelial cells and thus increases lung edema clearance.

Original languageEnglish (US)
Pages (from-to)626-633
Number of pages8
JournalAmerican journal of respiratory and critical care medicine
Issue number2
StatePublished - 1999

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine


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