Dopamine stimulates sodium transport and liquid clearance in rat lung epithelium

Michele L. Barnard, Walter C. Olivera, David M. Rutschman, Alejandro M. Bertorello, A. I. Katz, Jacob I. Sznajder*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

76 Scopus citations

Abstract

Pulmonary edema clearance is driven primarily by active sodium transport out of the alveoli, mediated predominantly by apical sodium channels and the basolateral NA,K-ATPase. We postulated that dopamine, analogous to its effects in other transporting epithelia, could regulate these sodium transport mechanisms and affect lung liquid clearance. We therefore studied the effects of dopamine on sodium transport and liquid clearance in isolated perfused rat lungs. Instillation of dopamine into the airways caused a dosedependent increase in liquid clearance from isolated rat lungs of up to 33% above control values at 10-8 to 10-4 M concentrations. 10-6 M amiloride, which selectively inhibits apical sodium channels, decreased basal liquid clearance by 34% but did not inhibit the dopaminemediated stimulation of lung liquid clearance. Instillation of 10-4 M amiloride into rat airways, which inhibits other Sodium transport mechanisms non-selectively, decreased basal lung liquid clearance by 49% and inhibited the dopamine- mediated stimulation of lung liquid clearance. Perfusion of rat lungs with 5 x 10-4 M ouabain to specifically inhibit Na,K-ATPase reduced both basal clearance (by 55%) and the dopamine-stimulated increase in lung fluid clearance. Conceivably, the stimulation of lung liquid clearance by dopamine is due to a modulation of Na,K-ATPase in the pulmonary epithelium.

Original languageEnglish (US)
Pages (from-to)709-714
Number of pages6
JournalAmerican journal of respiratory and critical care medicine
Volume156
Issue number3 I
DOIs
StatePublished - 1997

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

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