Double-antibody radioimmunoassay for factor VIII-related antigen

D. Green, N. Reynolds

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


A plasma protein required for the support of ristocetin-induced platelet aggregation was isolated from anti-hemophilic factor concentrate and radiolabeled with 125I. A double-antibody radioimmunoassay was developed, with use of specific rabbit anti-VIII related antigen serum and goat anti-rabbit globulin. The assay is sensitive, reproducible, and technically simple to perform. Values obtained in normal subjects ranged from 0.65 to 1.53 units, similar to our normal range for VIII coagulant activity (0.67-1.43 units). However, normal or increased values of VIII-related antigen were observed in VIII coagulant-deficient hemophiliacs. Also, concentrations of VIII-related antigen significantly exceeded coagulant concentrations in several patients with liver disease or disseminated intravascular coagulation, or both. Of a broad selection of congenital coagulation disorders examined, only patients with von Willebrand's disease had decreased VIII-related antigen concentrations, and these corresponded to the lowered concentration of ristocetin cofactor in the patients. In 3 transfused patients, VIII-related antigen values correlated with the concentration of the cofactor. Our results suggest that the radioimmunoassay of VIII-related antigen is a simple and valuable adjunct in the study of patients with clotting abnormalities.

Original languageEnglish (US)
Pages (from-to)1648-1653
Number of pages6
JournalClinical chemistry
Issue number9
StatePublished - 1977

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical


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