Double stranded DNA sequencing by tandem mass spectrometry

Fred W. McLafferty; David J. Aaserud; Ziqiang Guan; Daniel P. Little; Neil L. Kelleher

doi:10.1016/s0168-1176(97)00171-7

Double stranded DNA sequencing by tandem mass spectrometry

Fred W. McLafferty^*, David J. Aaserud, Ziqiang Guan, Daniel P. Little, Neil L. Kelleher

^*Corresponding author for this work

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Electrospray ionization produces far more abundant molecular ions for double stranded (ds) DNA than for single stranded (ss) and accurate molecular masses can provide the base composition of dsDNA. This study shows that the Fourier-transform mass spectra of a ds 64-mer DNA can provide approximately 50% more sequence information than the spectra of either individual ss component. Cleavages triggered by T loss are of low probability, but the opposite is true in the complementary ss strand that bears the base A at this site. The spectrum of the product of the attempted biological synthesis of a ds 70-mer DNA provided critical information on its extensive 3′-end heterogeneity. Published by Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	457-466
Number of pages	10
Journal	International Journal of Mass Spectrometry and Ion Processes
Volume	165-166
DOIs	https://doi.org/10.1016/s0168-1176(97)00171-7
State	Published - 1997

Keywords

DNA
Electrospray
Fourier-transform MS
Sequencing
Tandem

ASJC Scopus subject areas

Spectroscopy

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10.1016/s0168-1176(97)00171-7

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title = "Double stranded DNA sequencing by tandem mass spectrometry",

abstract = "Electrospray ionization produces far more abundant molecular ions for double stranded (ds) DNA than for single stranded (ss) and accurate molecular masses can provide the base composition of dsDNA. This study shows that the Fourier-transform mass spectra of a ds 64-mer DNA can provide approximately 50% more sequence information than the spectra of either individual ss component. Cleavages triggered by T loss are of low probability, but the opposite is true in the complementary ss strand that bears the base A at this site. The spectrum of the product of the attempted biological synthesis of a ds 70-mer DNA provided critical information on its extensive 3′-end heterogeneity. Published by Elsevier Science B.V.",

keywords = "DNA, Electrospray, Fourier-transform MS, Sequencing, Tandem",

author = "McLafferty, {Fred W.} and Aaserud, {David J.} and Ziqiang Guan and Little, {Daniel P.} and Kelleher, {Neil L.}",

note = "Funding Information: We thank Takeshi Sano for the Klenow fragment extension to prepare the 70-mer ds DNA and the National Institutes of Health (GM 16609) for generous financial support.",

year = "1997",

doi = "10.1016/s0168-1176(97)00171-7",

language = "English (US)",

volume = "165-166",

pages = "457--466",

journal = "International Journal of Mass Spectrometry and Ion Processes",

issn = "1387-3806",

publisher = "Elsevier",

}

TY - JOUR

T1 - Double stranded DNA sequencing by tandem mass spectrometry

AU - McLafferty, Fred W.

AU - Aaserud, David J.

AU - Guan, Ziqiang

AU - Little, Daniel P.

AU - Kelleher, Neil L.

N1 - Funding Information: We thank Takeshi Sano for the Klenow fragment extension to prepare the 70-mer ds DNA and the National Institutes of Health (GM 16609) for generous financial support.

PY - 1997

Y1 - 1997

N2 - Electrospray ionization produces far more abundant molecular ions for double stranded (ds) DNA than for single stranded (ss) and accurate molecular masses can provide the base composition of dsDNA. This study shows that the Fourier-transform mass spectra of a ds 64-mer DNA can provide approximately 50% more sequence information than the spectra of either individual ss component. Cleavages triggered by T loss are of low probability, but the opposite is true in the complementary ss strand that bears the base A at this site. The spectrum of the product of the attempted biological synthesis of a ds 70-mer DNA provided critical information on its extensive 3′-end heterogeneity. Published by Elsevier Science B.V.

AB - Electrospray ionization produces far more abundant molecular ions for double stranded (ds) DNA than for single stranded (ss) and accurate molecular masses can provide the base composition of dsDNA. This study shows that the Fourier-transform mass spectra of a ds 64-mer DNA can provide approximately 50% more sequence information than the spectra of either individual ss component. Cleavages triggered by T loss are of low probability, but the opposite is true in the complementary ss strand that bears the base A at this site. The spectrum of the product of the attempted biological synthesis of a ds 70-mer DNA provided critical information on its extensive 3′-end heterogeneity. Published by Elsevier Science B.V.

KW - DNA

KW - Electrospray

KW - Fourier-transform MS

KW - Sequencing

KW - Tandem

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U2 - 10.1016/s0168-1176(97)00171-7

DO - 10.1016/s0168-1176(97)00171-7

M3 - Article

AN - SCOPUS:0000865193

VL - 165-166

SP - 457

EP - 466

JO - International Journal of Mass Spectrometry and Ion Processes

JF - International Journal of Mass Spectrometry and Ion Processes

SN - 1387-3806

ER -

Double stranded DNA sequencing by tandem mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

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