Abstract
Electrospray ionization produces far more abundant molecular ions for double stranded (ds) DNA than for single stranded (ss) and accurate molecular masses can provide the base composition of dsDNA. This study shows that the Fourier-transform mass spectra of a ds 64-mer DNA can provide approximately 50% more sequence information than the spectra of either individual ss component. Cleavages triggered by T loss are of low probability, but the opposite is true in the complementary ss strand that bears the base A at this site. The spectrum of the product of the attempted biological synthesis of a ds 70-mer DNA provided critical information on its extensive 3′-end heterogeneity. Published by Elsevier Science B.V.
Original language | English (US) |
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Pages (from-to) | 457-466 |
Number of pages | 10 |
Journal | International Journal of Mass Spectrometry and Ion Processes |
Volume | 165-166 |
DOIs | |
State | Published - 1997 |
Keywords
- DNA
- Electrospray
- Fourier-transform MS
- Sequencing
- Tandem
ASJC Scopus subject areas
- Spectroscopy