Double-stranded RNA activates RANTES gene transcription through co-operation of nuclear factor-κB and interferon regulatory factors in human airway epithelial cells

K. Ieki, S. Matsukura*, F. Kokubu, T. Kimura, H. Kuga, M. Kawaguchi, M. Odaka, S. Suzuki, S. Watanabe, H. Takeuchi, R. P. Schleimer, M. Adachi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Background: Regulated on activation, normal T cells expressed and secreted (RANTES) is a member of the CC chemokine family and contributes to viral-induced airway inflammation including exacerbations of asthma. Double-stranded RNA (dsRNA) is known to be synthesized during replication of many viruses and a ligand of Toll-like receptor 3. We hypothesized that dsRNA may mimic viral infection and induce RANTES expression in airway epithelial cells. Objective: We first confirmed that dsRNA up-regulated RANTES mRNA and protein synthesis in the airway epithelial cells. We next focused our studies on the transcriptional regulation of RANTES. Methods: Airway epithelial cell line BEAS-2B and normal human bronchial epithelial cells were used in vitro study. Levels of RANTES mRNA and protein expression were determined with RT-PCR and ELISA. Mechanisms of transcriptional regulation were assessed by electrophoretic mobility shift assay and dual luciferase assay using RANTES promoter-luciferase reporter plasmids. Results: Activation of nuclear factor-κB (NF-κB) was confirmed by nuclear protein binding to a DNA probe derived from the RANTES promoter. Activity of the RANTES promoter was increased by dsRNA. The stimulation with dsRNA was partially inhibited in plasmids mutated at either of the binding sites for NF-κB or IFN regulatory factors (IRFs). When both sites were mutated, the activation was totally abrogated. Conclusion: These results imply that dsRNA activates NF-κB and IRFs and these transcription factors activate transcription of the RANTES promoter and its protein expression in airway epithelial cells.

Original languageEnglish (US)
Pages (from-to)745-752
Number of pages8
JournalClinical and Experimental Allergy
Volume34
Issue number5
DOIs
StatePublished - May 2004

Keywords

  • Asthma
  • Double-stranded RNA
  • Epithelial cells
  • IFN regulatory factor
  • NF-κB
  • RANTES
  • Toll-like receptor
  • Viral infection

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint

Dive into the research topics of 'Double-stranded RNA activates RANTES gene transcription through co-operation of nuclear factor-κB and interferon regulatory factors in human airway epithelial cells'. Together they form a unique fingerprint.

Cite this