Doublecortin is necessary for the migration of adult subventricular zone cells from neurospheres

Polloneal J. Ocbina, Maria L.V. Dizon, Laura Shin, Francis G. Szele*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Mutations in human doublecortin (DCX) and knockdown of Dcx in rodents cause radial migration defects in the embryonic cerebral cortex. However, the brain phenotype of Dcx knockout mice is largely normal suggesting that Dcx is not necessary for most migration events. Adult subventricular zone (SVZ) cells migrate tangentially in the rostral migratory stream to the olfactory bulbs. Dcx is expressed in the SVZ but it is unknown if it is necessary for migration. We show that Dcx RNAi reduced SVZ cell migration in vitro, both cell autonomously and non-cell autonomously. Overexpression of Dcx rescued migration after knockdown, but did not increase migration by itself. Thus, Dcx is necessary not only for embryonic radial migration but also migration of adult SVZ cells.

Original languageEnglish (US)
Pages (from-to)126-135
Number of pages10
JournalMolecular and Cellular Neuroscience
Volume33
Issue number2
DOIs
StatePublished - Oct 2006

Keywords

  • Cytoskeleton
  • Doublecortin
  • Migration
  • Neurospheres
  • Subependymal zone
  • Videomicroscopy

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Fingerprint Dive into the research topics of 'Doublecortin is necessary for the migration of adult subventricular zone cells from neurospheres'. Together they form a unique fingerprint.

Cite this