Doublecortin is necessary for the migration of adult subventricular zone cells from neurospheres

Polloneal J. Ocbina; Maria L.V. Dizon; Laura Shin; Francis G. Szele

doi:10.1016/j.mcn.2006.06.014

Doublecortin is necessary for the migration of adult subventricular zone cells from neurospheres

Polloneal J. Ocbina, Maria L.V. Dizon, Laura Shin, Francis G. Szele^*

^*Corresponding author for this work

Pediatrics

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Mutations in human doublecortin (DCX) and knockdown of Dcx in rodents cause radial migration defects in the embryonic cerebral cortex. However, the brain phenotype of Dcx knockout mice is largely normal suggesting that Dcx is not necessary for most migration events. Adult subventricular zone (SVZ) cells migrate tangentially in the rostral migratory stream to the olfactory bulbs. Dcx is expressed in the SVZ but it is unknown if it is necessary for migration. We show that Dcx RNAi reduced SVZ cell migration in vitro, both cell autonomously and non-cell autonomously. Overexpression of Dcx rescued migration after knockdown, but did not increase migration by itself. Thus, Dcx is necessary not only for embryonic radial migration but also migration of adult SVZ cells.

Original language	English (US)
Pages (from-to)	126-135
Number of pages	10
Journal	Molecular and Cellular Neuroscience
Volume	33
Issue number	2
DOIs	https://doi.org/10.1016/j.mcn.2006.06.014
State	Published - Oct 2006

Keywords

Cytoskeleton
Doublecortin
Migration
Neurospheres
Subependymal zone
Videomicroscopy

ASJC Scopus subject areas

Molecular Biology
Cellular and Molecular Neuroscience
Cell Biology

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10.1016/j.mcn.2006.06.014

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@article{eb187ee81ed54ee98efa0e3b0ab0b300,

title = "Doublecortin is necessary for the migration of adult subventricular zone cells from neurospheres",

abstract = "Mutations in human doublecortin (DCX) and knockdown of Dcx in rodents cause radial migration defects in the embryonic cerebral cortex. However, the brain phenotype of Dcx knockout mice is largely normal suggesting that Dcx is not necessary for most migration events. Adult subventricular zone (SVZ) cells migrate tangentially in the rostral migratory stream to the olfactory bulbs. Dcx is expressed in the SVZ but it is unknown if it is necessary for migration. We show that Dcx RNAi reduced SVZ cell migration in vitro, both cell autonomously and non-cell autonomously. Overexpression of Dcx rescued migration after knockdown, but did not increase migration by itself. Thus, Dcx is necessary not only for embryonic radial migration but also migration of adult SVZ cells.",

keywords = "Cytoskeleton, Doublecortin, Migration, Neurospheres, Subependymal zone, Videomicroscopy",

author = "Ocbina, {Polloneal J.} and Dizon, {Maria L.V.} and Laura Shin and Szele, {Francis G.}",

note = "Funding Information: The authors would like to thank Joe LoTurco for the Dcx RNAi plasmids. We thank Honglin Li and Christine DiDonato for advice throughout the project. We would also like to thank, Hans-Georg Simon, Jill Morris, and members of the Szele laboratory for critical readings of the manuscript. FGS supported by NIH RO1 NS/AG42253. ",

year = "2006",

month = oct,

doi = "10.1016/j.mcn.2006.06.014",

language = "English (US)",

volume = "33",

pages = "126--135",

journal = "Molecular and Cellular Neurosciences",

issn = "1044-7431",

publisher = "Academic Press Inc.",

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TY - JOUR

T1 - Doublecortin is necessary for the migration of adult subventricular zone cells from neurospheres

AU - Ocbina, Polloneal J.

AU - Dizon, Maria L.V.

AU - Shin, Laura

AU - Szele, Francis G.

N1 - Funding Information: The authors would like to thank Joe LoTurco for the Dcx RNAi plasmids. We thank Honglin Li and Christine DiDonato for advice throughout the project. We would also like to thank, Hans-Georg Simon, Jill Morris, and members of the Szele laboratory for critical readings of the manuscript. FGS supported by NIH RO1 NS/AG42253.

PY - 2006/10

Y1 - 2006/10

N2 - Mutations in human doublecortin (DCX) and knockdown of Dcx in rodents cause radial migration defects in the embryonic cerebral cortex. However, the brain phenotype of Dcx knockout mice is largely normal suggesting that Dcx is not necessary for most migration events. Adult subventricular zone (SVZ) cells migrate tangentially in the rostral migratory stream to the olfactory bulbs. Dcx is expressed in the SVZ but it is unknown if it is necessary for migration. We show that Dcx RNAi reduced SVZ cell migration in vitro, both cell autonomously and non-cell autonomously. Overexpression of Dcx rescued migration after knockdown, but did not increase migration by itself. Thus, Dcx is necessary not only for embryonic radial migration but also migration of adult SVZ cells.

AB - Mutations in human doublecortin (DCX) and knockdown of Dcx in rodents cause radial migration defects in the embryonic cerebral cortex. However, the brain phenotype of Dcx knockout mice is largely normal suggesting that Dcx is not necessary for most migration events. Adult subventricular zone (SVZ) cells migrate tangentially in the rostral migratory stream to the olfactory bulbs. Dcx is expressed in the SVZ but it is unknown if it is necessary for migration. We show that Dcx RNAi reduced SVZ cell migration in vitro, both cell autonomously and non-cell autonomously. Overexpression of Dcx rescued migration after knockdown, but did not increase migration by itself. Thus, Dcx is necessary not only for embryonic radial migration but also migration of adult SVZ cells.

KW - Cytoskeleton

KW - Doublecortin

KW - Migration

KW - Neurospheres

KW - Subependymal zone

KW - Videomicroscopy

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AN - SCOPUS:33748918604

VL - 33

SP - 126

EP - 135

JO - Molecular and Cellular Neurosciences

JF - Molecular and Cellular Neurosciences

SN - 1044-7431

IS - 2

ER -

Doublecortin is necessary for the migration of adult subventricular zone cells from neurospheres

Abstract

Keywords

ASJC Scopus subject areas

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