Abstract
Mutations in human doublecortin (DCX) and knockdown of Dcx in rodents cause radial migration defects in the embryonic cerebral cortex. However, the brain phenotype of Dcx knockout mice is largely normal suggesting that Dcx is not necessary for most migration events. Adult subventricular zone (SVZ) cells migrate tangentially in the rostral migratory stream to the olfactory bulbs. Dcx is expressed in the SVZ but it is unknown if it is necessary for migration. We show that Dcx RNAi reduced SVZ cell migration in vitro, both cell autonomously and non-cell autonomously. Overexpression of Dcx rescued migration after knockdown, but did not increase migration by itself. Thus, Dcx is necessary not only for embryonic radial migration but also migration of adult SVZ cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 126-135 |
| Number of pages | 10 |
| Journal | Molecular and Cellular Neuroscience |
| Volume | 33 |
| Issue number | 2 |
| DOIs | |
| State | Published - Oct 2006 |
Funding
The authors would like to thank Joe LoTurco for the Dcx RNAi plasmids. We thank Honglin Li and Christine DiDonato for advice throughout the project. We would also like to thank, Hans-Georg Simon, Jill Morris, and members of the Szele laboratory for critical readings of the manuscript. FGS supported by NIH RO1 NS/AG42253.
Keywords
- Cytoskeleton
- Doublecortin
- Migration
- Neurospheres
- Subependymal zone
- Videomicroscopy
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience
- Molecular Biology
- Cell Biology