Down-regulation of CFTR expression in an HT29-derived cell line by stable antisense RNA production

Catherine J C Wardle; Ann Harris

doi:10.1016/0167-4781(95)00043-G

Down-regulation of CFTR expression in an HT29-derived cell line by stable antisense RNA production

Catherine J C Wardle, Ann Harris^*

^*Corresponding author for this work

Pediatrics

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

In order to examine the interactions of the cAMP activated CFTR chloride ion channel with other chloride ion channels present in epithelial cells we have generated a model based on antisense mRNA down regulation of CFTR expression. The HT29-derived epithelial cell line CJC4-1 has integrated a plasmid that is constitutively expressing CFTR exons 1-6 in the antisense orientation, from the MoMULV LTR. This cell line shows a reduction in cAMP-activated chloride efflux, as measured by iodide efflux assay, in comparison to the parental HT29 cell line and a sense transfected control.

Original language	English (US)
Pages (from-to)	417-423
Number of pages	7
Journal	BBA - Gene Structure and Expression
Volume	1261
Issue number	3
DOIs	https://doi.org/10.1016/0167-4781(95)00043-G
State	Published - Apr 26 1995

Funding

We thank Drs. Barry Argent, Mike Gray and colleagues for helpful discussions; also Prof. E.R. Moxon for continued support; Dr. H. Land and Dr. P. Berg for plasmids. This work was funded by the Cystic Fibrosis Research Trust, UK.

Keywords

(HT29-derived cell line)
CFTR expression
Cystic fibrosis
Downregulation
antisense mRNA

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0167-4781(95)00043-G

Cite this

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title = "Down-regulation of CFTR expression in an HT29-derived cell line by stable antisense RNA production",

abstract = "In order to examine the interactions of the cAMP activated CFTR chloride ion channel with other chloride ion channels present in epithelial cells we have generated a model based on antisense mRNA down regulation of CFTR expression. The HT29-derived epithelial cell line CJC4-1 has integrated a plasmid that is constitutively expressing CFTR exons 1-6 in the antisense orientation, from the MoMULV LTR. This cell line shows a reduction in cAMP-activated chloride efflux, as measured by iodide efflux assay, in comparison to the parental HT29 cell line and a sense transfected control.",

keywords = "(HT29-derived cell line), CFTR expression, Cystic fibrosis, Downregulation, antisense mRNA",

author = "Wardle, {Catherine J C} and Ann Harris",

note = "Funding Information: We thank Drs. Barry Argent, Mike Gray and colleagues for helpful discussions; also Prof. E.R. Moxon for continued support; Dr. H. Land and Dr. P. Berg for plasmids. This work was funded by the Cystic Fibrosis Research Trust, UK. Copyright: Copyright 2015 Elsevier B.V., All rights reserved.",

year = "1995",

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doi = "10.1016/0167-4781(95)00043-G",

language = "English (US)",

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T1 - Down-regulation of CFTR expression in an HT29-derived cell line by stable antisense RNA production

AU - Wardle, Catherine J C

AU - Harris, Ann

N1 - Funding Information: We thank Drs. Barry Argent, Mike Gray and colleagues for helpful discussions; also Prof. E.R. Moxon for continued support; Dr. H. Land and Dr. P. Berg for plasmids. This work was funded by the Cystic Fibrosis Research Trust, UK. Copyright: Copyright 2015 Elsevier B.V., All rights reserved.

PY - 1995/4/26

Y1 - 1995/4/26

N2 - In order to examine the interactions of the cAMP activated CFTR chloride ion channel with other chloride ion channels present in epithelial cells we have generated a model based on antisense mRNA down regulation of CFTR expression. The HT29-derived epithelial cell line CJC4-1 has integrated a plasmid that is constitutively expressing CFTR exons 1-6 in the antisense orientation, from the MoMULV LTR. This cell line shows a reduction in cAMP-activated chloride efflux, as measured by iodide efflux assay, in comparison to the parental HT29 cell line and a sense transfected control.

AB - In order to examine the interactions of the cAMP activated CFTR chloride ion channel with other chloride ion channels present in epithelial cells we have generated a model based on antisense mRNA down regulation of CFTR expression. The HT29-derived epithelial cell line CJC4-1 has integrated a plasmid that is constitutively expressing CFTR exons 1-6 in the antisense orientation, from the MoMULV LTR. This cell line shows a reduction in cAMP-activated chloride efflux, as measured by iodide efflux assay, in comparison to the parental HT29 cell line and a sense transfected control.

KW - (HT29-derived cell line)

KW - CFTR expression

KW - Cystic fibrosis

KW - Downregulation

KW - antisense mRNA

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Down-regulation of CFTR expression in an HT29-derived cell line by stable antisense RNA production

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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