Down-regulation of CFTR expression in an HT29-derived cell line by stable antisense RNA production

Catherine J C Wardle, Ann Harris*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

In order to examine the interactions of the cAMP activated CFTR chloride ion channel with other chloride ion channels present in epithelial cells we have generated a model based on antisense mRNA down regulation of CFTR expression. The HT29-derived epithelial cell line CJC4-1 has integrated a plasmid that is constitutively expressing CFTR exons 1-6 in the antisense orientation, from the MoMULV LTR. This cell line shows a reduction in cAMP-activated chloride efflux, as measured by iodide efflux assay, in comparison to the parental HT29 cell line and a sense transfected control.

Original languageEnglish (US)
Pages (from-to)417-423
Number of pages7
JournalBBA - Gene Structure and Expression
Volume1261
Issue number3
DOIs
StatePublished - Apr 26 1995

Keywords

  • (HT29-derived cell line)
  • CFTR expression
  • Cystic fibrosis
  • Downregulation
  • antisense mRNA

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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