Downregulation of cPLA2γ expression inhibits EGF-induced chemotaxis of human breast cancer cells through Akt pathway

Gang Tian, Xueqian Wang, Fei Zhang, Hua Geng, Wei Hou, Liwei Chen, Hua Guo*, Ning Zhang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Phospholipids play an important role in mediating cell migration. In the present study, we investigated the role of cPLA2γ in chemotaxis of human breast cancer cells. Inhibition of cPLA2γ expression by small interference RNA severely inhibits EGF-induced chemotaxis in a dose-dependent manner in MDA-MB-231, MCF-7, T47D and ZR-75-30 cells. Furthermore, silencing cPLA2γ expression also impaired directional migration, adhesion and invasion in MDA-MB-231 cells. In addition, we investigated the molecular mechanism by which cPLA2γ regulated migration. Knockdown of cPLA2γ suppressed the phosphorylation of Akt at both Thr308 and Ser473. Phosphorylation of PKCζ, downstream of Akt, was also dampened. Knockdown of cPLA2γ also impaired the phosphorylation of integrin β1 and cofilin, key regulators of cell adhesion and actin polymerization, respectively. Taken together, our results suggest that cPLA2γ plays an important role in cancer cell chemotaxis.

Original languageEnglish (US)
Pages (from-to)506-512
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume409
Issue number3
DOIs
StatePublished - Jun 10 2011

Keywords

  • Akt
  • Breast cancer
  • CPLAγ
  • Chemotaxis
  • EGF

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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