Doxazosin-induced clusterin expression and apoptosis in prostate cancer cells

Yun Hee Youm; Hyunwon Yang; Yong Dal Yoon; Duk Yoon Kim; Chung Lee; Tag Keun Yoo

doi:10.1016/j.urolonc.2007.02.010

Doxazosin-induced clusterin expression and apoptosis in prostate cancer cells

Yun Hee Youm, Hyunwon Yang, Yong Dal Yoon, Duk Yoon Kim, Chung Lee, Tag Keun Yoo^*

^*Corresponding author for this work

Urology

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The purpose of this study was to correlate temporal expression of clusterin and apoptosis in androgen-independent human prostate cancer cells (PC-3) treated with 25 μM doxazosin. DNA fragmentation, reverse transcriptase polymerase chain reaction, and terminal transferase-mediated biotinylated 16-desoxy-uridene triphosphate nick-end labeling (TUNEL) assays were used to assess degree of apoptosis and temporal and spatial expression of clusterin mRNA and protein. DNA fragmentation was significant at 48 hours. Clusterin mRNA expression was 3-fold higher than control at 9 hours and was maintained over 48 hours. The TUNEL assay showed increasing percentage of apoptotic cells and presence of clusterin after doxazosin treatment. During doxazosin-induced apoptosis in PC3 cells, clusterin appeared to initially accumulate in the cytoplasm and protect against apoptosis; later, after its transport to the nucleus, clusterin was no longer able to suppress apoptosis.

Original language	English (US)
Pages (from-to)	483-488
Number of pages	6
Journal	Urologic Oncology: Seminars and Original Investigations
Volume	25
Issue number	6
DOIs	https://doi.org/10.1016/j.urolonc.2007.02.010
State	Published - Nov 2007

Keywords

Apoptosis
Clusterin
PC-3 cells
Prostate cancer cells

ASJC Scopus subject areas

Oncology
Urology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.urolonc.2007.02.010

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@article{7da503dc6a004655b5408421a91e056c,

title = "Doxazosin-induced clusterin expression and apoptosis in prostate cancer cells",

abstract = "The purpose of this study was to correlate temporal expression of clusterin and apoptosis in androgen-independent human prostate cancer cells (PC-3) treated with 25 μM doxazosin. DNA fragmentation, reverse transcriptase polymerase chain reaction, and terminal transferase-mediated biotinylated 16-desoxy-uridene triphosphate nick-end labeling (TUNEL) assays were used to assess degree of apoptosis and temporal and spatial expression of clusterin mRNA and protein. DNA fragmentation was significant at 48 hours. Clusterin mRNA expression was 3-fold higher than control at 9 hours and was maintained over 48 hours. The TUNEL assay showed increasing percentage of apoptotic cells and presence of clusterin after doxazosin treatment. During doxazosin-induced apoptosis in PC3 cells, clusterin appeared to initially accumulate in the cytoplasm and protect against apoptosis; later, after its transport to the nucleus, clusterin was no longer able to suppress apoptosis.",

keywords = "Apoptosis, Clusterin, PC-3 cells, Prostate cancer cells",

author = "Youm, {Yun Hee} and Hyunwon Yang and Yoon, {Yong Dal} and Kim, {Duk Yoon} and Chung Lee and Yoo, {Tag Keun}",

note = "Funding Information: This study was supported by grants from Pfizer Inc., Korea Research Foundation, and 2003 Eulji Research Grant (EJRG-03-007-11E24). ",

year = "2007",

month = nov,

doi = "10.1016/j.urolonc.2007.02.010",

language = "English (US)",

volume = "25",

pages = "483--488",

journal = "Urologic Oncology",

issn = "1078-1439",

publisher = "Elsevier Inc.",

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TY - JOUR

T1 - Doxazosin-induced clusterin expression and apoptosis in prostate cancer cells

AU - Youm, Yun Hee

AU - Yang, Hyunwon

AU - Yoon, Yong Dal

AU - Kim, Duk Yoon

AU - Lee, Chung

AU - Yoo, Tag Keun

N1 - Funding Information: This study was supported by grants from Pfizer Inc., Korea Research Foundation, and 2003 Eulji Research Grant (EJRG-03-007-11E24).

PY - 2007/11

Y1 - 2007/11

N2 - The purpose of this study was to correlate temporal expression of clusterin and apoptosis in androgen-independent human prostate cancer cells (PC-3) treated with 25 μM doxazosin. DNA fragmentation, reverse transcriptase polymerase chain reaction, and terminal transferase-mediated biotinylated 16-desoxy-uridene triphosphate nick-end labeling (TUNEL) assays were used to assess degree of apoptosis and temporal and spatial expression of clusterin mRNA and protein. DNA fragmentation was significant at 48 hours. Clusterin mRNA expression was 3-fold higher than control at 9 hours and was maintained over 48 hours. The TUNEL assay showed increasing percentage of apoptotic cells and presence of clusterin after doxazosin treatment. During doxazosin-induced apoptosis in PC3 cells, clusterin appeared to initially accumulate in the cytoplasm and protect against apoptosis; later, after its transport to the nucleus, clusterin was no longer able to suppress apoptosis.

AB - The purpose of this study was to correlate temporal expression of clusterin and apoptosis in androgen-independent human prostate cancer cells (PC-3) treated with 25 μM doxazosin. DNA fragmentation, reverse transcriptase polymerase chain reaction, and terminal transferase-mediated biotinylated 16-desoxy-uridene triphosphate nick-end labeling (TUNEL) assays were used to assess degree of apoptosis and temporal and spatial expression of clusterin mRNA and protein. DNA fragmentation was significant at 48 hours. Clusterin mRNA expression was 3-fold higher than control at 9 hours and was maintained over 48 hours. The TUNEL assay showed increasing percentage of apoptotic cells and presence of clusterin after doxazosin treatment. During doxazosin-induced apoptosis in PC3 cells, clusterin appeared to initially accumulate in the cytoplasm and protect against apoptosis; later, after its transport to the nucleus, clusterin was no longer able to suppress apoptosis.

KW - Apoptosis

KW - Clusterin

KW - PC-3 cells

KW - Prostate cancer cells

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SN - 1078-1439

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JO - Urologic Oncology

JF - Urologic Oncology

IS - 6

ER -

Doxazosin-induced clusterin expression and apoptosis in prostate cancer cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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