Doxazosin-induced clusterin expression and apoptosis in prostate cancer cells

Yun Hee Youm, Hyunwon Yang, Yong Dal Yoon, Duk Yoon Kim, Chung Lee, Tag Keun Yoo*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The purpose of this study was to correlate temporal expression of clusterin and apoptosis in androgen-independent human prostate cancer cells (PC-3) treated with 25 μM doxazosin. DNA fragmentation, reverse transcriptase polymerase chain reaction, and terminal transferase-mediated biotinylated 16-desoxy-uridene triphosphate nick-end labeling (TUNEL) assays were used to assess degree of apoptosis and temporal and spatial expression of clusterin mRNA and protein. DNA fragmentation was significant at 48 hours. Clusterin mRNA expression was 3-fold higher than control at 9 hours and was maintained over 48 hours. The TUNEL assay showed increasing percentage of apoptotic cells and presence of clusterin after doxazosin treatment. During doxazosin-induced apoptosis in PC3 cells, clusterin appeared to initially accumulate in the cytoplasm and protect against apoptosis; later, after its transport to the nucleus, clusterin was no longer able to suppress apoptosis.

Original languageEnglish (US)
Pages (from-to)483-488
Number of pages6
JournalUrologic Oncology: Seminars and Original Investigations
Volume25
Issue number6
DOIs
StatePublished - Nov 1 2007

Keywords

  • Apoptosis
  • Clusterin
  • PC-3 cells
  • Prostate cancer cells

ASJC Scopus subject areas

  • Oncology
  • Urology

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