TY - JOUR
T1 - DQ 65-79, A Peptide Derived from HLA Class II, Mimics p21 to Block T Cell Proliferation
AU - Dong, Chen
AU - Lyu, Shu Chen
AU - Krensky, Alan M.
AU - Clayberger, Carol
PY - 2003/11/15
Y1 - 2003/11/15
N2 - DQ 65-79, a peptide derived from residues 65-79 of the α-chain HLA class II molecule DQA03011, blocks T cell proliferation and induces T cell apoptosis. Using a yeast two-hybrid assay, we previously identified proliferating cell nuclear Ag (PCNA) as an intracellular ligand for DQ 65-79. In this study, we show that three regions of PCNA, residues 81-100, 121-140, and 241-261, interact with DQ 65-79. Residues 241-261 of PCNA also interact with the C terminus (residues 139-160) of the cell cycle regulator, p21, suggesting that DQ 65-79 and p21 might function similarly. We show here that DQ 65-79 competitively inhibits binding of p21 to PCNA and that both DQ 65-79 and p21 139-160 induce T cell apoptosis, suggesting that DQ 65-79 and p21 act similarly to inhibit cell growth.
AB - DQ 65-79, a peptide derived from residues 65-79 of the α-chain HLA class II molecule DQA03011, blocks T cell proliferation and induces T cell apoptosis. Using a yeast two-hybrid assay, we previously identified proliferating cell nuclear Ag (PCNA) as an intracellular ligand for DQ 65-79. In this study, we show that three regions of PCNA, residues 81-100, 121-140, and 241-261, interact with DQ 65-79. Residues 241-261 of PCNA also interact with the C terminus (residues 139-160) of the cell cycle regulator, p21, suggesting that DQ 65-79 and p21 might function similarly. We show here that DQ 65-79 competitively inhibits binding of p21 to PCNA and that both DQ 65-79 and p21 139-160 induce T cell apoptosis, suggesting that DQ 65-79 and p21 act similarly to inhibit cell growth.
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U2 - 10.4049/jimmunol.171.10.5064
DO - 10.4049/jimmunol.171.10.5064
M3 - Article
C2 - 14607903
AN - SCOPUS:0242580044
VL - 171
SP - 5064
EP - 5070
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 10
ER -