DQ 65-79, A Peptide Derived from HLA Class II, Mimics p21 to Block T Cell Proliferation

Chen Dong, Shu Chen Lyu, Alan M. Krensky, Carol Clayberger*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

DQ 65-79, a peptide derived from residues 65-79 of the α-chain HLA class II molecule DQA03011, blocks T cell proliferation and induces T cell apoptosis. Using a yeast two-hybrid assay, we previously identified proliferating cell nuclear Ag (PCNA) as an intracellular ligand for DQ 65-79. In this study, we show that three regions of PCNA, residues 81-100, 121-140, and 241-261, interact with DQ 65-79. Residues 241-261 of PCNA also interact with the C terminus (residues 139-160) of the cell cycle regulator, p21, suggesting that DQ 65-79 and p21 might function similarly. We show here that DQ 65-79 competitively inhibits binding of p21 to PCNA and that both DQ 65-79 and p21 139-160 induce T cell apoptosis, suggesting that DQ 65-79 and p21 act similarly to inhibit cell growth.

Original languageEnglish (US)
Pages (from-to)5064-5070
Number of pages7
JournalJournal of Immunology
Volume171
Issue number10
DOIs
StatePublished - Nov 15 2003

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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