Driving proteins off DNA using applied tension

John F. Marko; Eric D. Siggia

doi:10.1016/S0006-3495(97)78248-1

Driving proteins off DNA using applied tension

John F. Marko^*, Eric D. Siggia

^*Corresponding author for this work

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

92 Scopus citations

Abstract

Proteins that bind DNA so as to reduce its end-to-end length can be dissociated by application of force. The thermodynamics of this process are discussed, with special attention to the case of histories bound to DNA (i.e., a string of nucleosomes, or chromatin fiber). The histone octamer is predicted to be driven off chromatin fiber for tensions >2 piconewtons.

Original language	English (US)
Pages (from-to)	2173-2178
Number of pages	6
Journal	Biophysical Journal
Volume	73
Issue number	4
DOIs	https://doi.org/10.1016/S0006-3495(97)78248-1
State	Published - Oct 1997

ASJC Scopus subject areas

Biophysics

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10.1016/S0006-3495(97)78248-1

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abstract = "Proteins that bind DNA so as to reduce its end-to-end length can be dissociated by application of force. The thermodynamics of this process are discussed, with special attention to the case of histories bound to DNA (i.e., a string of nucleosomes, or chromatin fiber). The histone octamer is predicted to be driven off chromatin fiber for tensions >2 piconewtons.",

author = "Marko, {John F.} and Siggia, {Eric D.}",

note = "Funding Information: The authors thank J. Widom, C. Bustamante, and S. Leuba for many helpful discussions. This work was supported by National Science Foundation Grant DMR 9121654 (to E.D.S.).",

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AU - Marko, John F.

AU - Siggia, Eric D.

N1 - Funding Information: The authors thank J. Widom, C. Bustamante, and S. Leuba for many helpful discussions. This work was supported by National Science Foundation Grant DMR 9121654 (to E.D.S.).

PY - 1997/10

Y1 - 1997/10

N2 - Proteins that bind DNA so as to reduce its end-to-end length can be dissociated by application of force. The thermodynamics of this process are discussed, with special attention to the case of histories bound to DNA (i.e., a string of nucleosomes, or chromatin fiber). The histone octamer is predicted to be driven off chromatin fiber for tensions >2 piconewtons.

AB - Proteins that bind DNA so as to reduce its end-to-end length can be dissociated by application of force. The thermodynamics of this process are discussed, with special attention to the case of histories bound to DNA (i.e., a string of nucleosomes, or chromatin fiber). The histone octamer is predicted to be driven off chromatin fiber for tensions >2 piconewtons.

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Driving proteins off DNA using applied tension

Abstract

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