Drosophila CK2 phosphorylates Hairy and regulates its activity in vivo

Bhaskar Kahali, Regina Trott, Ze'ev Paroush, Ravi Allada, Clifton P. Bishop, Ashok P. Bidwai*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Hairy is a repressor that regulates bristle patterning, and its loss elicits ectopic bristles (neural hyperplasia). However, it has remained unknown whether Hairy is regulated by phosphorylation. We describe here the interaction of protein kinase CK2 and Hairy. Hairy is robustly phosphorylated by the CK2-holoenzyme (CK2-HoloE) purified from Drosophila embryos, but weakly by the catalytic CK2α-subunit alone, suggesting that this interaction requires the regulatory CK2β-subunit. Consistent with this, Hairy preferentially forms a direct complex with CK2-HoloE. Importantly, we demonstrate genetic interactions between CK2 and hairy (h). Thus, flies trans-heterozygous for alleles of CK2α and h display neural hyperplasia akin to homozygous hypomorphic h alleles. In addition, we show that similar phenotypes are elicited in wild-type flies upon expression of RNAi constructs against CK2α/β, and that these defects are sensitive to h gene dosage. Together, these studies suggest that CK2 contributes to repression by Hairy.

Original languageEnglish (US)
Pages (from-to)637-642
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume373
Issue number4
DOIs
StatePublished - Sep 5 2008

Keywords

  • CK2
  • Drosophila
  • Hairy
  • Neurogenesis
  • Repression

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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