Drosophila larval NMJ immunohistochemistry

Jonathan Robert Brent*, Kristen Werner, Brian D. McCabe

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The Drosophila neuromuscular junction (NMJ) is an established model system used for the study of synaptic development and plasticity. The widespread use of the Drosophila motor system is due to its high accessibility. It can be analyzed with single-cell resolution. There are 30 muscles per hemisegment whose arrangement within the peripheral body wall are known. A total of 31 motor neurons attach to these muscles in a pattern that has high fidelity. Using molecular biology and genetics, one can create transgenic animals or mutants. Then, one can study the developmental consequences on the morphology and function of the NMJ. Immunohistochemistry can be used to clearly image the components of the NMJ. In this article, we demonstrate how to use antibody staining to visualize the Drosophila larval NMJ.

Original languageEnglish (US)
Article numbere1108
JournalJournal of Visualized Experiments
Issue number25
DOIs
StatePublished - Mar 2009

Keywords

  • Developmental biology
  • Drosophila
  • Immunohistochemistry
  • Issue 25
  • Larvae
  • NMJ
  • Neuroscience

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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