Abstract
Drosophila S2 cells and mammalian CHO-K1 cells were used to investigate the requirements for HSV-1 cell fusion. Infection assays indicated S2 cells were not permissive for HSV-1. HVEM and nectin-1 mediated cell fusion between CHO-K1 cells and S2 cells when either CHO-K1 or S2 cells were used as target cells. Interestingly, PILRα did not mediate fusion between CHO-K1 or S2 cells due to a glycosylation defect of PILRα and gB in S2 cells. Fusion activity was not detected for any receptor tested when S2 cells were used both as target cells and effector cells indicating S2 cells may lack a key cellular factor present in mammalian cells that is required for cell fusion. Thus, insect cells may provide a novel tool to study the interaction of HSV-1 glycoproteins and cellular factors required for fusion, as well as a means to identify unknown cellular factors required for HSV replication.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 100-109 |
| Number of pages | 10 |
| Journal | Virology |
| Volume | 437 |
| Issue number | 2 |
| DOIs | |
| State | Published - Mar 15 2013 |
Funding
We appreciate the generous gifts from Gary H. Cohen and Roselyn J. Eisenberg for R45 and R137 antibodies, from Dr. Carl F. Ware for the plasmid of FLAG-HVEM, from Gregory A. Smith for fluorescent viruses vGS2843 and vGS2822, and from Dr. Y. Takai for plasmid of FLAG-nectin-1. We thank N. Susmarski for timely and excellent technical assistance and members of the Longnecker Laboratory for their help in these studies. Traditional sequencing services were performed at the Northwestern University Genomics Core Facility. RL is the Dan and Bertha Spear Research Professor in Microbiology—Immunology. This work was supported by NIH Grants CA021776 and AI067048 to RL . KPB was supported by the training program in Immunology and Molecular Pathogenesis from the National Institutes of Health ( T32AI07476 ).
Keywords
- CHO-K1 cells
- Drosophila S2 cells
- Fusion activity
- HSV-1
ASJC Scopus subject areas
- Virology