TY - JOUR
T1 - Dual-band optical coherence tomography using a single supercontinuum laser source
AU - Chen, Siyu
AU - Shu, Xiao
AU - Yi, Ji
AU - Fawzi, Amani
AU - Zhang, Hao F.
N1 - Funding Information:
This work is supported in part by the following agencies: National Institutes of Health (1R01EY019951, 1R24EY022883, 1DP3DK108248) and National Science Foundation (CBET- 1055379, DBI-1353952). H. F. Zhang has financial interests in Opticent Health, which, however, did not support this work.
Publisher Copyright:
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE).
PY - 2016/6/1
Y1 - 2016/6/1
N2 - We developed a simultaneous visible-light (Vis) and near-infrared (NIR) dual-band optical coherence tomography (OCT) system using a single supercontinuum laser source. The goal was to benchmark our newly developed Vis-OCT against the well-developed NIR-OCT. The Vis-OCT subsystem operated at 91 nm full-width-at-half-maximum (FWHM) bandwidth centered at 566 nm; the NIR-OCT subsystem operated at 93 nm FWHM bandwidth centered at 841 nm. The axial resolutions were 1.8 and 4.4 μm in air for the Vis- and NIR-OCT subsystems, respectively. We compared the respective performances, including anatomical imaging, angiography, absolute retinal blood flow measurements, and spectroscopic analysis for retinal blood oxygen saturation (sO2), between the two subsystems in rodents in vivo. While demonstrating minor discrepancies related to operation wavelengths, both subsystems showed comparable performances in the first three tests. However, we were only able to retrieve sO2 using the Vis-OCT subsystem.
AB - We developed a simultaneous visible-light (Vis) and near-infrared (NIR) dual-band optical coherence tomography (OCT) system using a single supercontinuum laser source. The goal was to benchmark our newly developed Vis-OCT against the well-developed NIR-OCT. The Vis-OCT subsystem operated at 91 nm full-width-at-half-maximum (FWHM) bandwidth centered at 566 nm; the NIR-OCT subsystem operated at 93 nm FWHM bandwidth centered at 841 nm. The axial resolutions were 1.8 and 4.4 μm in air for the Vis- and NIR-OCT subsystems, respectively. We compared the respective performances, including anatomical imaging, angiography, absolute retinal blood flow measurements, and spectroscopic analysis for retinal blood oxygen saturation (sO2), between the two subsystems in rodents in vivo. While demonstrating minor discrepancies related to operation wavelengths, both subsystems showed comparable performances in the first three tests. However, we were only able to retrieve sO2 using the Vis-OCT subsystem.
KW - dual-band
KW - optical coherence tomography
KW - optical coherence tomography flowmetry
KW - optical coherence tomography oximetry
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U2 - 10.1117/1.JBO.21.6.066013
DO - 10.1117/1.JBO.21.6.066013
M3 - Article
C2 - 27304421
AN - SCOPUS:84975526666
SN - 1083-3668
VL - 21
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
IS - 6
M1 - 066013
ER -