Duramycin radiosensitization of MCA-RH 7777 hepatoma cells through the elevation of reactive oxygen species

Bowen Yang; Xiaoke Huang; Weiguo Li; Samdeep Mouli; Robert Lewandowski; Andrew Larson

doi:10.4103/jcrt.JCRT_284_18

Duramycin radiosensitization of MCA-RH 7777 hepatoma cells through the elevation of reactive oxygen species

Bowen Yang, Xiaoke Huang, Weiguo Li, Samdeep Mouli, Robert Lewandowski, Andrew Larson^*

^*Corresponding author for this work

Radiology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Objective: The objective of this study is to explore the radiosensitization effects of duramycin against the liver cancer hepatoma cells and relationship to reactive oxygen species (ROS) generation. Materials and Methods: MCA-RH 7777 cells were treated with various combinations of duramycin concentrations and radiation doses. After the treatment, cell viabilities were determined by a cell proliferation assay; intracellular ROS levels were detected with the flow cytometric method. Results: MCA-RH 7777 cell viability was found significantly reduced after combining duramycin and radiation exposure (comparing to that of either treatment alone). Increased intracellular ROS levels were observed in cells treated with combinations of duramycin and radiation. Conclusion: Duramycin increased the intracellular ROS generation and also increased the radiosensitivity of MCA-RH 7777 cells.

Original language	English (US)
Pages (from-to)	543-546
Number of pages	4
Journal	Journal of Cancer Research and Therapeutics
Volume	17
Issue number	2
DOIs	https://doi.org/10.4103/jcrt.JCRT_284_18
State	Published - Apr 1 2021

Funding

This study was supported by NIH NCI RO1CA181658.

Keywords

Duramycin
radiosensitivity
reactive oxygen species

ASJC Scopus subject areas

Oncology
Radiology Nuclear Medicine and imaging

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.4103/jcrt.JCRT_284_18

Cite this

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title = "Duramycin radiosensitization of MCA-RH 7777 hepatoma cells through the elevation of reactive oxygen species",

abstract = "Objective: The objective of this study is to explore the radiosensitization effects of duramycin against the liver cancer hepatoma cells and relationship to reactive oxygen species (ROS) generation. Materials and Methods: MCA-RH 7777 cells were treated with various combinations of duramycin concentrations and radiation doses. After the treatment, cell viabilities were determined by a cell proliferation assay; intracellular ROS levels were detected with the flow cytometric method. Results: MCA-RH 7777 cell viability was found significantly reduced after combining duramycin and radiation exposure (comparing to that of either treatment alone). Increased intracellular ROS levels were observed in cells treated with combinations of duramycin and radiation. Conclusion: Duramycin increased the intracellular ROS generation and also increased the radiosensitivity of MCA-RH 7777 cells.",

keywords = "Duramycin, radiosensitivity, reactive oxygen species",

author = "Bowen Yang and Xiaoke Huang and Weiguo Li and Samdeep Mouli and Robert Lewandowski and Andrew Larson",

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doi = "10.4103/jcrt.JCRT_284_18",

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T1 - Duramycin radiosensitization of MCA-RH 7777 hepatoma cells through the elevation of reactive oxygen species

AU - Yang, Bowen

AU - Huang, Xiaoke

AU - Li, Weiguo

AU - Mouli, Samdeep

AU - Lewandowski, Robert

AU - Larson, Andrew

PY - 2021/4/1

Y1 - 2021/4/1

N2 - Objective: The objective of this study is to explore the radiosensitization effects of duramycin against the liver cancer hepatoma cells and relationship to reactive oxygen species (ROS) generation. Materials and Methods: MCA-RH 7777 cells were treated with various combinations of duramycin concentrations and radiation doses. After the treatment, cell viabilities were determined by a cell proliferation assay; intracellular ROS levels were detected with the flow cytometric method. Results: MCA-RH 7777 cell viability was found significantly reduced after combining duramycin and radiation exposure (comparing to that of either treatment alone). Increased intracellular ROS levels were observed in cells treated with combinations of duramycin and radiation. Conclusion: Duramycin increased the intracellular ROS generation and also increased the radiosensitivity of MCA-RH 7777 cells.

AB - Objective: The objective of this study is to explore the radiosensitization effects of duramycin against the liver cancer hepatoma cells and relationship to reactive oxygen species (ROS) generation. Materials and Methods: MCA-RH 7777 cells were treated with various combinations of duramycin concentrations and radiation doses. After the treatment, cell viabilities were determined by a cell proliferation assay; intracellular ROS levels were detected with the flow cytometric method. Results: MCA-RH 7777 cell viability was found significantly reduced after combining duramycin and radiation exposure (comparing to that of either treatment alone). Increased intracellular ROS levels were observed in cells treated with combinations of duramycin and radiation. Conclusion: Duramycin increased the intracellular ROS generation and also increased the radiosensitivity of MCA-RH 7777 cells.

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KW - radiosensitivity

KW - reactive oxygen species

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Duramycin radiosensitization of MCA-RH 7777 hepatoma cells through the elevation of reactive oxygen species

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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